薯蓣皂苷对培养乳鼠心肌细胞缺氧／复氧损伤的保护作用

目的研究薯蓣皂苷对培养乳鼠心肌细胞缺氧／复氧损伤的保护作用并探讨其作用机制。方法采用体外培养乳鼠心肌细胞，建立缺氧／复氧损伤模型，以薯蓣皂苷进行干预。心肌细胞随机分为5组：空白对照组（C组）；缺氧／复氧组（A／R组）；薯蓣皂苷（Dioscin）低、中、高剂量干预组（Dioscin＋A／R组）。分别观察各组心肌细胞搏动频率；MTr法测细胞存活率；用Rhl23荧光探针标记线粒体，检测荧光强度以反映线粒体膜电位（△ψm）变化；Fluo-3负载心肌细胞，激光扫描共聚焦显微镜观察细胞内钙离子浓度变化。结果薯蓣皂苷干预组心肌细胞搏动频率、细胞存活率、△ψm与A／R组比较明显升高；细胞内平均钙离子荧光强度显著低于A／R组。结论从细胞水平初步证实薯蓣皂苷预处理心肌细胞对A／R损伤有一定保护作用，保护机制可能涉及对线粒体膜保护和防止细胞内钙超载等。

The Protective Effect of Dioscin on Cultured Cardiomyocytesin Anoxia/Reoxygenation Injury

Objective To investigate the mechanisms of A/R and the effects of dioscin on the cul- tured myocardial cells. Methods Cultured myocardial cells of neonatal Wistar rats were randomly divided into five groups： Control group; A/R group; dioscin L, M, H group. At the end of experiment, myocardial cells beating rate, cell viability, alteration of mitochondria membrane potential （△ψm） on myocardial cells were determined by Rh123 staining method, alteration of Ca^2＋ ] i on myocardial cells were determined by LSCM. Result Compared with A/R group , the beating rate, cell viability and △ψm of each dioscin group was significantly increased. Compared with AZR group, the average fluorescence intensity of Ca^2＋ ] i of each dioscin group was significantly reduced. Conclusion It is suggested that dioscin may be involved in the process of protection on cultured myocardial cells in A/R injury. The protection of mitochondria membrane and prevention of calcium overload may be involved in the mechanism of dioscin-protection.