| 林蛙肠道中乳酸菌的分离及鉴定 |
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| 引用本文： | 王开艳,朱明秋,李艳茹,张宗植,周末,白雪梅,李太元. 林蛙肠道中乳酸菌的分离及鉴定[J]. 水产科学, 2009, 28(8) |
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| 作者姓名： | 王开艳 朱明秋 李艳茹 张宗植 周末 白雪梅 李太元 |
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| 作者单位： | 1. 延边大学,农学院,吉林,龙井,133400
2. 延吉市畜牧兽医局,吉林,延吉,133000 |
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| 摘 要： | 用2种方法从健康林蛙肠道菌群中分离乳酸菌,并进一步纯化及鉴定.试验结果表明,用乳酸菌半选择性培养基液体培养液先富集后分离得到了11株单一的乳酸杆菌,经过一系列生化鉴定,11株菌为同一种菌即嗜酸乳杆菌.该菌对嗜水气单胞菌具有抑制作用,抑菌圈直径为12 mm.在胆盐含量为0.3%,pH值为3时仍然生长,表明该菌株完全能适应动物胃肠道环境,作为微生态制剂用菌种具有较好的潜力.
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| 关 键 词： | 林蛙 乳酸菌 分离 鉴定 |
Isolation and Identification of Lactobacillus from Intestinal Tract of Wood Frog |
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| WANG Kai-yan,ZHU Ming-qiu,LI Yan-ru,ZHANG Zong-zhi,ZHOU Mo,BAI Xue-mei,LI Tai-yuan. Isolation and Identification of Lactobacillus from Intestinal Tract of Wood Frog[J]. Fisheries Science, 2009, 28(8) |
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| Authors: | WANG Kai-yan ZHU Ming-qiu LI Yan-ru ZHANG Zong-zhi ZHOU Mo BAI Xue-mei LI Tai-yuan |
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| Affiliation: | 1.Microbiotic Laboratory;Yanbian University Longjing;133400;China;2.Animal Husbandry and Veterinary Medicine Institute;Yanji 133000;China |
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| Abstract: | Based on the microecological principle,lactic acid bacteria were isolated from the intestinal tract of healthy wood frog by two kinds of isolation methods and purified and identified further.The results showed that all of the eleven bacteria isolated after richment by MRS liquid culture were pure Lactobacillus.After a series of biochemical identification,the eleven bacteria were the same strain,which is L.acidophilus.This Lactobacillus was shown to inhibit Aeromonas,and the inhibition zone was 12 mm.It stil... |
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| Keywords: | wood frog Lactobacillus isolation characterization |
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