尿液中利尿剂、丙磺舒、咖啡因、匹莫林的固相提取和HPLC初筛分析

A solid-phase extraction and reversed phase high performance liquidchromatographic method(RP-HPLC) was developed for the rapid determination of 13 diuretics (bel-onging to five different pharmacological groups) ,probenecid, caffeine and pemoline in urine. Two mlurine sample was first adsorbed on a XAD-2 column, then eluted with ether--ethyl acetate(l: 1).The eluate was evaporated to dryness and reconstituted in methanol. The methanolic solution wasinjected into a HP LiChrosorb RP-18 column, using phosphate buffer (pH 3) and acetonitrile as the mo-bile phase and monitored at 216 nm, 230 nm, and 275 nm on a diode array ultraviolet detector. Theextraction recoveries of 16 drugs were above 75%. The limits of detection ranged from 0. 3～3.0μg/ml of urine. All drugs were separately administered to healthy volunteers, positive urine sampleswere collected, and urinary excretion--time curves of some drugs were reported.

SOLID-PHASE EXTRACTION AND RP-HPLC SCREENING ROCEDURE FOR DIURETICS,PROBENECID,CAFFEINE AND PEMOLINE IN URINE

A solid-phase extraction and reversed phase high performance liquid chromatographic method (RP-HPLC) was developed for the rapid determination of 13 diuretics (belonging to five different pharmacological groups), probenecid, caffeine and pemoline in urine. Two ml urine sample was first adsorbed on a XAD-2 column, then eluted with ether-ethyl acetate (1:1). The eluate was evaporated to dryness and reconstituted in methanol. The methanolic solution was injected into a HP LiChrosorb RP-18 column, using phosphate buffer (pH 3) and acetonitrile as the mobile phase and monitored at 216 nm, 230 nm, and 275 nm on a diode array ultraviolet detector. The extraction recoveries of 16 drugs were above 75%. The limits of detection ranged from 0.3-3.0 micrograms/ml of urine. All drugs were separately administered to healthy volunteers, positive urine samples were collected, and urinary excretion-time curves of some drugs were reported.