| 应用染色体G显带技术评估辐射剂量和染色体畸变的量效关系 |
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| 引用本文： | 施常备,陈晓泉,姚俊涛,袁勇,赵征,袁彬,赵明刚,陈葳,王翔.应用染色体G显带技术评估辐射剂量和染色体畸变的量效关系[J].癌变．畸变．突变,2011,23(2):134-136. |
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| 作者姓名： | 施常备 陈晓泉 姚俊涛 袁勇 赵征 袁彬 赵明刚 陈葳 王翔 |
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| 作者单位： | 施常备,陈晓泉,姚俊涛,袁勇,赵征,袁彬,SHI Chang-bei,CHEN Xiao-quan,YAO Jun-tao,YUAN Yong,ZHAO Zheng,YUAN Bin(陕西省肿瘤医院,陕西,西安,710061);赵明刚,陈葳,王翔,ZHAO Minggang,CHEN Wei,WANG Xiang(西安交通大学第一附属医院,陕西,西安,710061) |
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| 基金项目： | 2010年陕西省卫生厅科研基金项目 |
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| 摘 要： | 目的：应用染色体G显带技术观察4MV的X线辐射后的染色体畸变情况,分析不同类型的染色体畸变和辐射剂量的关系。方法：以4MV的X线0.5、1、2、4Gy对离体人外周血进行辐照,其淋巴细胞培养后,采用染色体G显带分析不同类型的染色体畸变率。结果：染色体双着丝粒、缺失、易位以及片段畸变和辐射剂量呈线性正相关,其线性趋势线和R~2值依次分别为：Y=24.1X-13.3（R~2=0.975）;Y=10.5X-2.7（R~2=0.887）;Y=30.2X-17.8（R~2=0.913）;Y=53.3X-38.7（R~2=0.976）。结论：染色体G显带技术可以较非显带技术提供更多更准确的畸变信息,为进一步研究提供借鉴。
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| 关 键 词： | 辐射 染色体畸变 G显带技术 |
The value of G-banding chromosomal assay in ionizing-radiation-induced chromosomal aberration |
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| SHI Chang-bei,CHEN Xiao-quan,YAO Jun-tao,YUAN Yong,ZHAO Zheng,YUAN Bin,ZHAO Minggang,CHEN Wei,WANG Xiang.The value of G-banding chromosomal assay in ionizing-radiation-induced chromosomal aberration[J].Carcinogenesis,Teratogenesis and Mutagenesis,2011,23(2):134-136. |
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| Authors: | SHI Chang-bei CHEN Xiao-quan YAO Jun-tao YUAN Yong ZHAO Zheng YUAN Bin ZHAO Minggang CHEN Wei WANG Xiang |
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| Affiliation: | (2,*) (1.Shaanxi Tumor Hospital,Xi’an 710061,Shaanxi;2.First Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710061,Shaanxi,China) |
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| Abstract: | OBJECTIVE:To validate the value of G-banding chromosomal assay in ionizing-radiation-induced chromosomal aberration.METHODS:Blood was irradiated with 0.5,1,2 and 4Gy,by 4 MV X-ray.The G-banding chromosomal aberration was assayed including dicentrics,deletions,translocations,rings,and fragments of chromosomes.RESULTS:Chromosomal aberration rates of dicentrics,deletions,translocations,and fragments all showed linear increase with radiation dose,with correlations of Y=24.1X-13.3(R~2=0.975);Y=10.5X-2.7(R~2=0.887); Y=30.2X-17.8(R~2=0.913);Y=53.3X-38.7(R~2=0.976),respectively.CONCLUSION:G-banding chromosomal aberration assay deserve more attention in radiation biology. |
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| Keywords: | radiation chromosomal aberration assay G-banding technique |
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