牛心包脱细胞处理后免疫原性的研究

牛心包用0.5%胰蛋白酶处理3 h,探讨其作为组织工程心脏瓣膜支架材料的可行性。大鼠被分成新鲜牛心包组、脱细胞处理牛心包组、空白对照组三组,分别测定其血清中产生的特异性抗牛心包抗体IgG的OD值,结果发现IgG含量在术后7 d三组间比较无统计学意义(P>0.05),其余各时点间比较差异有显著性(P<0.05),在相应时点新鲜牛心包产生的IgG值比脱细胞处理的牛心包高,且新鲜牛心包产生的IgG值在术后2月达峰值,而脱细胞处理牛心包产生的IgG在术后1月达峰值。组织学观察发现脱细胞处理后牛心包完全清除了细胞成分,纤维网架结构轻度松散,但基本保持完整。结果表明,这种脱细胞方法能完全清除牛心包上的细胞成分,降低免疫原性。

Immunogenicity of Acellular Bovine Pericardium in Vivo

To investigate the feasibility of making up bovine pericardium into scaffolds for tissue engineering valve,it was decellularized by 0.5% trypsin incubation for three hours.The rats were divided into fresh bovine group,acellular pericardium group and control group,and OD value of rat anti-bovine IgG was measured.Serum levels of IgG showed no difference among the three groups on the 7~(th) day postoperatively(P>0.05).The OD values of IgG at other time-points displayed distinct difference among the three groups(P<0.05).Fresh bovine pericardium group produced more IgG than acellular bovine pericardium group did at the same time-point.The IgG level of fresh bovine pericardium group reached the peak postoperatively,but in acellular bovine pericardium group its peak value appeared on the 30~(th) day postoperatively.Histological examination demonstrated that the bovine pericardium was thoroughly decellularized,the fibrous structure was slightly loose,but remained essentially intact.These results indicate that the acellularization procedure can result in a complete removal of cells and decrease of immunogenicity.