一氧化氮,内皮素在急性坏死性胰腺炎肠道损伤中的作用

目的探讨一氧化氮（ＮＯ），内皮素１（ＥＴ１）在急性坏死性胰腺炎（ＡＮＰ）肠道损伤中的作用。方法制成犬ＡＮＰ模型。分别于术后第１，２，４，７ｄ测定血浆ＮＯ，ＥＴ１水平和二胺氧化酶（ＤＡＯ）活性，并对肠粘膜超微结构和肠道细菌移位情况进行观察。结果ＡＮＰ组犬血浆ＮＯ和ＥＴ１水平显著高于对照组，均以发病后第１，２ｄ最为明显（Ｐ＜０．０１～Ｐ＜０．０５）；血中细菌移位率为１００％，亦以发病后第１，２ｄ为最高（６／８，５／８）；肠粘膜微绒毛出现破损和部分脱落，面积减少。结论ＮＯ和ＥＴ１是导致ＡＮＰ时肠道损伤的重要因素，抑制ＥＴ１的产生和减少早期ＮＯ的超量释放，可减轻ＡＮＰ对肠道的损伤作用。

The effects of plasma nitric oxide, endothelin 1 on gut injury in acute necrotizing pancreatitis in dogs

Objective To investigate the effects of plasma nitric oxide(NO) and endothelin 1(ET 1) on gut injury in acute necrotizing pancreatitis(ANP). Methods After ANP model was induced in dogs, plasma levels of NO, ET 1 and diamine oxidase (DAO) were measured on the postoperative day 1,2,4,7. Bacterial translocation of blood and ultrastructure of mucosa were also observed. Results Plasma levels of NO and ET 1 were much higher in ANP group than that in control group. Bacterial translocation rate of ANP dogs was of 100%, and the positive blood cultare occurred on day 1, 2 postoperatively (75%, 62.5%) in major of the ANP dogs; epithelial villi of the mucosa was damaged, and the number of villi reduced. Conclusions As important factors NO and ET 1 are pare of gut injury in ANP, and inhibition of the production of ET 1 and NO may decrease the gut injury in ANP dogs.