Effects of Linoleic Acid on Conjugated Linoleic Acid Production by Planktonic Rumen Bacteria from Grain-fed Cows |
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| Authors: | Y J Kim KW Lee S Oh HJ Lee |
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| Affiliation: | Author Kim is with the Dept. of Molecular Biology and Genetics, Biotechnology Building, Cornell Univ., Ithaca, NY 14853–2703. Author K.W. Lee and H.J. Lee are with the Dept. of Food Science and Technology, School of Agricultural Biotechnology, Seoul Natl. Univ., Suwon, Korea. Author Oh is with the Korea Yakult Co. Ltd., Seoul, Korea. Direct inquiries to author Kim (E-mail: ). |
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| Abstract: | Ruminal conjugated linoleic acid (CLA) production from linoleic acid (LA) was characterized in vitro. Rumen bacteria from grain‐fed cows were more active in BH than those from hay‐fed cows. Particleassociated bacteria produced more hydrogenated products leaving less CLA than the planktonic bacteria (P < 0.05). CLA production by planktonic bacteria did not always correlate to LA given; longer incubations generally decreased CLA concentration and increased c9, t11/t10, c12 ratio, especially at higher LA concentrations. The preincubated cells to LA produced more CLA than the unexposed ones and the increase was more evident with c9, t11 CLA (P < 0.05). This study provides insight into how cattle diet and LA feedings affect ruminal CLA production. |
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| Keywords: | conjugated linoleic acid biohydrogenation linoleic acid planktonic bacteria rumen |
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