急性缺氧对豚鼠耳蜗热休克蛋白70表达的影响

目的探讨急性缺氧条件下豚鼠耳蜗热休克蛋白(HSP70)表达的变化。方法利用气管插管辅助呼吸并给予不同浓度低氧气体建立动物模型，利用免疫组化的方法，观察了在正常及不同程度的急性缺氧条件下，豚鼠耳蜗HSP70的表达。结果正常豚鼠耳蜗有HSP70的表达，主要分布在螺旋神经节、Corti’s器及齿间细胞，缺氧条件下上述各个部位HSP70的表达显著增强，增强的程度与缺氧程度无关。结论急性缺氧可以诱导豚鼠耳蜗HSP70表达增强，表达部位与正常耳蜗组织相同，不同程度的缺氧对其表达的强度没有影响。HSP70在豚鼠耳蜗的表达，可能对内耳组织的结构和功能有一定的保护作用。

Effects of Acute Hypoxia on Expression of Heat Shock Protein 70 in Cochlea of Guinea Pigs

Objective: To investigate the changes of the expression of heat shock protein 70 (HSP70) in the cochlea of guinea pigs under acute hypoxia. Method: Different degrees of acute hypoxia were imposed on guinea pigs and slices of the cochlea were examined by immunohistochemistry to detect HSP70. Result: In normal situation, HSP70 is expressed in guinea pig's cochlea, especially in spiral ganglion and Corti's organ, under acute hypoxia, this expression was strengthened, and the degree of this strengthening showed no correlation with degree of hypoxia. Conclusion: These results suggest that acute hypoxia can strengthen the expression of HSP70 in guinea pig's cochlea, and the degree of hypoxia has no effects on the degree of this strengthening. The expression of HSP70 in the cochlea may be a kind of protection to the structure and the function of the inner ear.