| 重离子辐照柠檬酸菌株的诱变选育 |
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| 引用本文： | 胡伟,陈积红,张珍,刘敬,王曙阳,李文建.重离子辐照柠檬酸菌株的诱变选育[J].辐射研究与辐射工艺学报,2012(1):53-57. |
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| 作者姓名： | 胡伟 陈积红 张珍 刘敬 王曙阳 李文建 |
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| 作者单位： | 甘肃农业大学食品科学与工程学院;中国科学院近代物理研究所 |
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| 基金项目： | 中国科学院科技支甘项目(KJZG-2011-002)资助 |
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| 摘 要： | 应用不同剂量重离子束12C+6对黑曲霉出发菌株4﹟进行辐照选育,选育出的高产酸菌株进行发酵试验,应用酸碱中和法测定发酵液中柠檬酸的含量。结果表明:当重离子12C+6的辐照剂量为60 Gy时,正突变率最大,为21%。诱变后的菌株通过酸斑法初筛、摇瓶复筛选育出1株菌株H4002,其发酵液中柠檬酸含量比出发菌株4﹟提高了18%。这说明利用重离子束12C+6辐照处理黑曲霉产生菌可以得到高产柠檬酸突变菌株,其诱变效果显著。
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| 关 键 词： | 黑曲霉 重离子束 诱变 选育 |
Mutation breeding of Aspergillus niger for producing citric acid by heavy ion beam |
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| HU Wei,CHEN Jihong,ZHANG Zhen,LIU Jing,WANG Shuyang,LI Wenjian.Mutation breeding of Aspergillus niger for producing citric acid by heavy ion beam[J].Journal of Radiation Research and Radiation Processing,2012(1):53-57. |
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| Authors: | HU Wei CHEN Jihong ZHANG Zhen LIU Jing WANG Shuyang LI Wenjian |
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| Affiliation: | 1(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730030,China) 2(Institute of Modern Physics,Chinese Academy of Science,Lanzhou 730000,China) |
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| Abstract: | In order to screen high-producing-citric acid strains,the original strain 4# was irradiated with different doses of heavy ion beams 12C+6.The citric acid content in the fermentation broth was measured by acid-base neutralization.The results showed that the positive mutation rate reached the highest value of 21% as the heavy ion 12C+6 irradiation dose was 60 Gy,and the high producing citric acid strain H4002 was obtained after screening.Compared with the original strain,its fermentation content of citric acid was improved by 18%.The results suggest that high-producing-citric acid strain could be obtained after it was irradiated by heavy ion beams and the mutation effect was remarkable. |
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| Keywords: | Aspergillus niger Heavy ion beam Mutation Breeding |
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