喉鼻咽重复癌中病毒感染初探

目的探讨Epstein-Barr病毒(EBV)、人乳头瘤病毒(HPV)在喉鼻咽重复癌中的感染状况。方法苏木素-伊红(HE)染色观察细胞形态,间接酶联免疫法检测EB病毒VCA抗体,以及原位杂交法检测EBER和HPV6/11、16/18、31/33。结果患者治疗前血浆EBV-VCAIGA阳性,鼻咽癌组织中存在大量EBV颗粒,而喉癌组织中偶见EBV感染,两种组织标本中均未见三型HPV。结论喉部上皮不是EBV易感上皮,重复癌可以存在不同的病毒感染状态。

Research on HPV and EBV Infection in multiple primary malignant tumors of nasopharynx and larynx in human

Objective To study the infection of EBV and HPV in a case of synchronous multiple primary malignant tumors of nasopharynx and larynx.Methods EBV-VCAIgA in plasma was detected by ELISA technique,and HPV6/11、16/18、31/33 and EBER in tissues were detected by hybridization in site.Results EBER was positive obviously in carcinoma of nasopharynx and positive occasionally in carcinoma of larynx.But three kinds of HPV were negative in both cancers.Plasma EBV-VCAIgA was present before treatment and disappeared after treatment.Conclusion Laryngeal tissue is not sensitive to EBV,and MPMT of nasopharynx and larynx is not caused by the same virus although their pathological locations are adjacent.