| Glucan在Con A所致免疫性肝损伤中的作用 |
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| 引用本文： | 马雪莲,高建新,李跃华,李菁,吴翠贞. Glucan在Con A所致免疫性肝损伤中的作用[J]. 中国生化药物杂志, 2005, 26(4): 215-217 |
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| 作者姓名： | 马雪莲 高建新 李跃华 李菁 吴翠贞 |
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| 作者单位： | 1. 山东大学医学院生理学研究所,山东济南250012
2. 南京医科大学病理学与病理生理学系,江苏南京210029 |
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| 摘 要： | 目的观察Glucan对刀豆蛋白A(ConA)所致小鼠免疫性肝损伤的干预效果及其作用机制。方法ConA尾静脉注射制备小鼠免疫性肝损伤模型,在ConA注射前1h腹腔注射Glucan,观察正常对照组、ConA损伤组、Glucan/ConA组肝损伤的情况,检测血清谷丙转氨酶(ALT)、α肿瘤坏死因子(TNF-α)及组织中一氧化氮(NO)含量的变化,并用DNAladder检测肝细胞凋亡的变化。结果ConA尾静脉注射后,出现明显的肝损伤,血清ALT、TNF-α及组织中NO水平明显升高,DNAladder检测可见明显的梯状条带;预先应用Glucan后,可减轻这些改变。结论Glu-can对ConA所致小鼠免疫性肝损伤有保护作用。
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| 关 键 词： | Glucan 肝脏 刀豆蛋白A 一氧化氮 α肿瘤坏死因子 |
| 文章编号： | 1005-1678(2005)04-0215-03 |
| 修稿时间： | 2004-10-21 |
The protective effect of Glucan on liver injury induced by Con A |
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| MA Xue-Lian,GAO Jian-xin,LI Yue-hua,LI Jing,WU Cui-zhen. The protective effect of Glucan on liver injury induced by Con A[J]. Chinese Journal of Biochemical Pharmaceutics, 2005, 26(4): 215-217 |
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| Authors: | MA Xue-Lian GAO Jian-xin LI Yue-hua LI Jing WU Cui-zhen |
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| Affiliation: | MA Xue-lian~1,GAO Jian-xin~1,LI Yue-hua~2,LI Jing~2,WU Cui-zhen~2 |
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| Abstract: | PurposeTo observe the effect and the mechanism of Glucan on concanavalin A (Con A)-induced liver injury.MethodsIn Con A group, Con A was administered iv via tail vein. In Glucan/Con A group, mice were pretreated with Glucan prior to the injury of Con A. The ALT, TNF-α levels in serum and the nitric oxide (NO) levels in liver tissue were determined in control and the treated groups. Apoptosis was determined by DNA ladder detection.ResultsALT, TNF-α levels in serum and the nitric oxide levels in liver tissue increased significantly after Con A injection. DNA ladder can be found. Pretreatment with Glucan could prevent these changes and lessen the Con A-induced liver injury.ConclusionGlucan has protective effect on Con A-induced liver injury. |
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| Keywords: | Glucan liver concanavalin A nitric oxide TNF-α |
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