| 荧光光谱法研究二氢卟吩e6和锌二氢卟吩e6与牛血清白蛋白的相互作用 |
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| 引用本文： | 韩露,秦军,罗一帆.荧光光谱法研究二氢卟吩e6和锌二氢卟吩e6与牛血清白蛋白的相互作用[J].应用化工,2011(10):1722-1726. |
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| 作者姓名： | 韩露 秦军 罗一帆 |
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| 作者单位： | 中山大学南方学院;格瑞恩新能源材料股份有限公司;华南师范大学化学与环境学院; |
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| 基金项目： | 广东省科技厅资助项目(2007FJ4152) |
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| 摘 要： | 应用紫外光谱和荧光光谱法研究二氢卟吩e6(Ce6)和锌二氢卟吩e6(ZnCe6)分别与牛血清白蛋白(BSA)的相互作用。通过紫外光谱、荧光光谱、光照实验,探讨了两种物质与BSA相互作用的荧光猝灭光谱特征、猝灭机理、与BSA结合反应及光照的影响。结果表明,Ce6和ZnCe6都可与BSA发生相互作用,Ce6和ZnCe6浓度的变化会引起Ce6、ZnCe6与BSA之间能量转移的变化,Ce6的猝灭程度要比ZnCe6强,猝灭机制是由静态猝灭引起的。Ce6与BSA的结合能力强于锌二氢卟吩e6,结合位点数均接近1,结合的作用力类型主要为范德华力和氢键作用力。Ce6-BSA和ZnCe6-BSA复合物均有光漂白特性,光漂白机制均为光学修饰型引起的,这种机制有利于提高光动力治疗(PDT)效果。
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| 关 键 词： | 荧光光谱 二氢卟吩e6 锌二氢卟吩e6 牛血清白蛋白 光漂白 |
Study on the interaction between chlorin e6,zinc clorin e6 and bovine serum albumin by fluorescence spectrophtometry |
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| HAN Lu,QIN Jun,LUO Yi-fan.Study on the interaction between chlorin e6,zinc clorin e6 and bovine serum albumin by fluorescence spectrophtometry[J].Applied chemical industry,2011(10):1722-1726. |
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| Authors: | HAN Lu QIN Jun LUO Yi-fan |
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| Affiliation: | HAN Lu1,QIN Jun2,LUO Yi-fan3(1.Nanfang College,Zhongshan University,Guangzhou 510970,China,2.Green New Energy Material INC,Xinxiang 453000,3.School of Chemistry and Environment,South China Normal University,Guangzhou 510006,China) |
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| Abstract: | The interaction between clorin e6(Ce6),zinc clorin e6(ZnCe6) and bovine serum albumin(BSA) were studied by fluorescence spectra.Under the condition of pH 7.4,each binding constants Kb and the binding sites n were obtained by fluorescence quenching.The themodynamic parameters were calculated to judge the mains orts of binding force fluorescence quenching of BSA by both Ce6 and ZnCe6 was deduced by static quenching.The binding constants of Ce6 were greater than that of ZnCe6,both their numbers of binding site... |
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| Keywords: | fluorescence spectroscopy clorin e6 zinc clorin e6 bovine aerum albumin photobleaching |
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