| 丙酸高产菌株的复合诱变选育 |
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| 引用本文： | 揣玉多,王德培,王艳萍,丁友昉. 丙酸高产菌株的复合诱变选育[J]. 食品与发酵工业, 2007, 33(6): 45-49 |
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| 作者姓名： | 揣玉多 王德培 王艳萍 丁友昉 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院,天津,300457 |
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| 摘 要： | 以费氏丙酸菌IFFI.10019为出发菌株,经2次紫外线、2次亚硝基胍、1次亚硝基胍-氯化锂多重复合诱变处理,选育获得丙酸高产菌株NL—3,丙酸产量由原来的0.20g/L,提高到1.23g/L,提高率达到515%。实验证明采用多因子复合诱变,特别是NTG-LiCl复合诱变,能有效改变菌株对诱变因素的敏感性,提高变异率,逐步提高突变株的产丙酸水平。
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| 关 键 词： | 丙酸 复合诱变 突变株 |
| 修稿时间： | 2006-11-232006-12-28 |
Compound Mutation Breeding of Propionic Acid High-yield Strain |
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| Chuai Yuduo,Wamg Depei,Wang Yanping,Ding Youfang. Compound Mutation Breeding of Propionic Acid High-yield Strain[J]. Food and Fermentation Industries, 2007, 33(6): 45-49 |
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| Authors: | Chuai Yuduo Wamg Depei Wang Yanping Ding Youfang |
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| Abstract: | Mutant(NL-3)Strain producting high-yield Propionic Acid was screened from Propionibacteria freudenreichii IFFI.10019 after treated with UV two times,NTG for two times and NTG-LiCl one time. the Propionic Acid production level reached up to 1.23 g/L,with an increase rate of 515% compared to 0.20 g/L from original strain.The experiments showed that using compound mutation can alter the original stain's sensitivity to mutagenic agents,especially NTG-LiCl,increase mutation frequency and raise Propionic Acid yield. |
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| Keywords: | Propionic Acid compound mutation mutant |
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