过氧化物酶体增殖物激活受体γ及其配体对早期细胞滋养细胞MMP-2、MMP-9表达的调控

目的:探讨过氧化物酶体增殖物激活型受体γ(PPARγ)及其配体(15-脱氧-前列腺素J2,15-d-PGJ2)对细胞滋养细胞表达基质金属蛋白酶-2(MMP-2)和MMP-9的调控作用。方法:采用免疫荧光细胞化学方法检测细胞滋养细胞中PPARγ的表达;利用免疫荧光共聚焦技术观察15-d-PGJ2作用前后细胞滋养细胞MMP-2和MMP-9表达强度的变化;通过荧光定量PCR(Real-timePCR)和Westernblot方法定量检测MMP-2和MMP-9mRNA和蛋白的表达变化。结果:在细胞滋养细胞中有PPARγ蛋白表达,且主要定位在细胞滋养细胞核中;15-d-PGJ2作用后细胞滋养细胞中MMP-2和MMP-9的表达明显下降,与对照组相比差异显著(P<0.01);15-d-PGJ2对MMP-2的作用强于MMP-9。结论:PPARγ及其配体15-d-PGJ2调节滋养细胞浸润作用可能是通过调节MMP-2和MMP-9的表达实现的。

Regulation of PPARγ and its Ligands 15-d-PGJ2 on the Expression of MMP-2 and MMP-9 in Cytotrophoblast in First Trimester

Objective: To investigate the influence of peroxisome proliferator-activated receptor gamma(PPARγ) and its ligand 15-deoxy-delta(12,14)-prostaglandin J2(15-d-PGJ2) on the expression of matrixmetalloproteinase(MMP)-2 and MMP-9 in cytotrophoblast in first trimester. Methods: The expression of PPARγ incytotrophoblast in first trimester was examined by immunocytochemistry, and the regulation of PPARγ ligands 15-d-PGJ2on the expression of MMP-2 and MMP-9 in cytotrophoblast was examined by confocal technique, Real-timequantitative PCR and Western blot. Results: PPARγ expression was detectable in the nucleus of cytotrophoblast cell.The expressions of MMP-9 and MMP-2 in cytotrophoblast cell were inhibited by 15-d-PGJ2 significantly (P<0.01),and in a concentration-dependent manner. Effect of 15-d-PGJ2 on the expression of MMP-2 was higher than thaton MMP-9. Conclusion: PPARγ ligand 15-d-PGJ2 can inhibit cytotrophoblast invasion through downregulatingexpressions of MMP-2 and MMP-9.