Treatment development for systemic Tetrahymena sp. infection in guppies,Poecilia reticulata Peters |
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Authors: | M Pimenta Leibowitz J Kumar Chettri R Ofir D Zilberg |
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Affiliation: | 1. The French Associates Institute for Agriculture and Biotechnology of Drylands, The Jacob Blaustein Institutes for Desert Research, Ben‐Gurion University of the Negev, Midreshet Ben‐Gurion, Israel;2. The Dead Sea and Arava Science Center, Central Arava Extension, Hatzeva, Israel;3. ;4. Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg C., Denmark;5. The Dead Sea and Arava Science Center, Central Arava Extension, Hatzeva, Israel |
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Abstract: | Antibacterial and antiparasitic agents and a cysteine protease inhibitor (E‐64) were tested against Tetrahymena infection, a serious problem in guppy production worldwide. Chemicals were tested in vitro by a colorimetric assay for Tetrahymena survival. The most effective were niclosamide, albendazole and chloroquine, with 23%, 35% and 60% survival, respectively, following 2‐h exposure to 100 ppm. Longer incubation periods resulted in greater reductions in survival. Niclosamide was further studied in vivo at different dosages, administered orally to Tetrahymena‐infected guppies. Mortality rates were significantly lower in all treatment groups; in trial I, 30% and 33% mortality in 5 and 40 mg kg?1 niclosamide‐fed fish vs. 59% mortality in controls; in trial II, 35%, 13% and 10% in 50, 100 and 200 mg kg?1 niclosamide‐fed fish vs. 64% in controls. The effect of the cysteine protease inhibitor E64 was tested in tissue culture, by measuring histolytic activity of the parasite (Tet‐NI) on a guppy‐fin cell line, based on cell depletion. Tet‐NI feeding activity was significantly reduced following pretreatment with E‐64 relative to non‐treated Tet‐NI. E‐64‐pretreated Tet‐NI was injected i.p. into guppies: recorded mortality rates were significantly lower (35%) than that in non‐treated Tet‐NI (60%), suggesting inhibition of the parasite’s cysteine protease as a possible therapeutic approach. |
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Keywords: | cysteine protease fish guppy infection Tetrahymena treatment |
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