肺灌洗液中表面活性物质和炎症因子测定判断胸外科手术致肺损伤

目的:通过检测支气管肺泡灌洗液(BALF)中肺泡表面活性物质(PS)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)及白细胞介素-8(IL-8)的含量研究开胸手术对肺造成的损伤及其机理。方法:开左胸行食管癌根治主动脉弓上吻合术患者24例,经纤维支气管镜灌洗肺泡(BAL),检测手术前后手术侧和非手术侧肺灌洗液(BALF)中PS组分和TNF-α、IL-1β和IL-8的变化,并同时检测手术前后血浆中上述细胞因子含量的变化。测定总磷脂(TPL)、饱和卵磷脂(SatPC)和总蛋白(TP)。取SatPC/TPL和SatPC/TP比值作为判断PS活性水平的指标。结果:术前左侧及右侧BALF中SatPC/TPL％和SatPC/TP％无显著差异(P>0.05);TNF-α(pmol·L~(-1)·mg~(-1)Pr)、IL-1β(μg·L~(-1)·mg~(-1)Pr)、IL-8(μg·L~(-1)·mg~(-1)Pr)含量均无显著差异(P>0.05)。手术后术侧肺SatPC/TPL及SatPC/TP值明显低于术前(30.15±11.91％ vs58 15±18.19％,P<0.01;6.54±2.94％ vs 12.28±2.44％,P<0.01),非手术侧SatPC/TPL及SatPC/TP比值无明显差异(P>0.05)手术侧肺术后BALF中TNF-α和IL-1β含量较术前明显上升(91.50±22.50 vs 45.80±11.69,P<0.01;0.89±0.24 vs 0.33±0.10,P<0.01),较对侧亦有明显上升(91.50±22.50 vs 51.90±11.99,P<0.01;0.89±0.24 vs0.38±0

Evaluation of Thoracotomy Induced Lung Injury by Assay of Pulmonary Sur-factant and Cytokines in Broncho- alveolar Lavage Fluid

Objective: To evaluate the thoracotomy induced lung injury by assay of in broncho - alveolar lavage fluid(BALF). Methods: Fourteen patients with esophageal carcinoma performed esophagectomy with anastomosis over the arcus aortae entered the study. Bilateral broncho - alveolar lavage (BAL) was carried out after intubation preoperatively and before the removal of the cannula postoperatively. Total phospholipid (TPL),saturated phosphatidylcholine(SatPC) and total protein(TP) in the broncho - alveolar lavage fluid(BALF) were measured. The ratio of SatPC/TPL% and SatPC/TP% represented the activity of PS. Results: Preoperatively, neither the ratio of SatPC/TPL% and SatPC/TP% nor the quantity of TNF - ? (pmol