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| 具有DNA切割功能的新型多聚酰胺/丝组缀合物 | |
| 引用本文： | 牛明玉,叶勇,刘伟,张祯,赵玉芬.具有DNA切割功能的新型多聚酰胺/丝组缀合物[J].高等学校化学学报,2006,27(7):1253-1256. |
| 作者姓名： | 牛明玉 叶勇 刘伟 张祯 赵玉芬 |
| 作者单位： | 1. 清华大学化学系,生命有机磷化学及化学生物学教育部重点实验室,北京,100084 2. 郑州大学化学系,化学生物学和有机化学河南省重点实验室,郑州,450052 3. 清华大学化学系,生命有机磷化学及化学生物学教育部重点实验室,北京,100084;郑州大学化学系,化学生物学和有机化学河南省重点实验室,郑州,450052 |
| 基金项目： | 国家自然科学基金(批准号:20132020,20572061)资助 |
| 摘 要： | 为得到具有核酸切割功能的人工核酸酶, 设计合成了一种新型多聚酰胺/丝组缀合物, 并研究了其DNA切割活性. 合成的目标化合物在pH=6.0的BR缓冲溶液中对pBR322 DNA切割活性的初步实验结果表明, 于37 ℃保温6 h后, pBR322 DNA基本上被完全从Form Ⅰ切割为Form Ⅱ, 保温36 h后, pBR322 DNA几乎被切割完全. |
| 关 键 词： | 多聚酰胺/丝组缀合物 DNA 切割试剂 |
| 文章编号： | 0251-0790（2006）07-1253-04 |
| 收稿时间： | 06 7 2005 12:00AM |
| 修稿时间： | 2005-06-07 |
A Novel Polyamide/Ser-His Conjugate with DNA-cleaving Function |
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| NIU Ming-Yu,YE Yong,LIU Wei,ZHANG Zhen,ZHAO Yu-Fen.A Novel Polyamide/Ser-His Conjugate with DNA-cleaving Function[J].Chemical Research In Chinese Universities,2006,27(7):1253-1256. | |
| Authors: | NIU Ming-Yu YE Yong LIU Wei ZHANG Zhen ZHAO Yu-Fen |
| Affiliation: | 1. Key laboratory of Bioorganic Phosphorus Chemistry &; Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China; 2. Department of Chemistry, Key Laboratory of Chemical Biology and Organic Chemistry, Zhengzhou University, Zhengzhou 450052, China |
| Abstract: | Recently, polyamides containing N-methylpyrrole have attracted considerable attention from synthetic and biological chemists. Based on Dervan's theory, the polyamide/Ser His conjugate which is a synthetic analogue of natural products for DNA affinity was synthesized through the dicyclohexylcarbodiimide/1-hydroxybenzotriazole(DCC/HOBt) coupling reaction, and its cleavage activity for DNA was also studied. In BR buffer(pH=60), pBR322 DNA can be cleavaged by the title compound at 37 ℃. After 6 h, pBR 322 DNA was transferred from form Ⅰ to form Ⅱ, after 36 h, pBR 322 DNA was cleavaged totally. |
| Keywords: | Polyamide/Ser-His conjugate DNA Cleavage agent |
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