厄贝沙坦对大鼠急性胰腺炎局部微循环改变的实验研究

目的 探讨血管紧张素受体拮抗剂对大鼠急性胰腺炎（AP）的保护作用.方法 40只Wistar 大鼠随机分为对照组和AP组,AP组分为模型组和高中低剂量组.测定血浆血管紧张素Ⅱ（AngⅡ）、淀粉酶（AMY）水平,观察胰腺组织病理学改变；采用异硫氰酸荧光素标记红细胞（FITC-RBC）技术及微循环观测系统观察大鼠胰腺微循环血液流速.结果 随着病变的进展,AP组胰腺炎病理由水肿向出血坏死发展,AngⅡ、AMY同步上升,胰腺局部组织血液流速降低.中剂量厄贝沙坦干预组则胰腺炎病理改变减轻,胰腺局部组织血液流速改善,且血浆AngⅡ、AMY水平低于模型组,其他剂量组之间各指标差异无统计学意义.结论血管紧张素受体拮抗剂对AP时胰腺微循环有明显的改善作用.

Experiments of local microcirculation change of lrbesartan on rats with acute pancreatitis

Objective To explore the angiotensin receptor antagonist protective effect on rat acute pancreatitis（AP）.Methods Forty Wistar rats were randomly divided into control group and group with AP,AP group was divided into model group and high school low-dose group.Determination of plasma angiotensin Ⅱ （Ang Ⅱ）,amylase （AMY） levels were observed histopathological changes of the pancreas ; fluorescein isothiocyanatelabeled red blood cells （FITC-RBC） technology and micro-circulation observing system observed in rat pancreatic microcirculation blood flow.Results As the lesions progress,in AP groups pancreatitis disease grounds edema to hemorrhage and necrosis development,Ang Ⅱ,AMY had been rising,pancreatic tissue blood flow rate decreases.Dose irbesartan intervention group pancreatitis alleviate the pathological changes of pancreatic tissue blood flow improved,and plasma Ang Ⅱ,AMY level lower than the model group,each index showed no significant difference between the other dose groups.Conclusions Angiotensin receptor antagonist significantly improve pancreatic microcirculation in AP.