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Supplementing culture and vitrification-warming media with l-ascorbic acid enhances survival rates and redox status of IVP porcine blastocysts via induction of GPX1 and SOD1 expression
Authors:Miriam Castillo-Martí  n,Sergi Bonet,Roser Morató  ,Marc Yeste
Affiliation:1. Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, E-17071 Girona, Spain;2. Unit of Animal Reproduction, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra (Barcelona), Spain
Abstract:
Keywords:AC, l-ascorbic acid   CNT, control   GPX1, glutathione peroxidase 1   IVC, in vitro culture   IVC-CNT, blastocysts cultured without AC   IVC-AC, blastocysts cultured with AC   IVP, in vitro produced   SOD1, superoxide dismutase 1   SOD2, superoxide dismutase 2   TCN, total cell number   VW, vitrification-warming   VW-CNT, blastocysts vitrified-warmed without AC   VW-AC, blastocysts vitrified-warmed with AC
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