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香菇的深层发酵工艺应用研究
引用本文:王谦,刘利群,闫蕾蕾,冀宏,陈文杰. 香菇的深层发酵工艺应用研究[J]. 河北大学学报(自然科学版), 2004, 24(2): 172-174. DOI: 10.3969/j.issn.1000-1565.2004.02.015
作者姓名:王谦  刘利群  闫蕾蕾  冀宏  陈文杰
作者单位:河北大学,生命科学学院,河北,保定,071002;河北省科学院,微生物研究所,河北,保定,071051
基金项目:河北省科技攻关项目 (0 2 2 2 0 15 0 4D)
摘    要:对香菇的发酵工艺研究表明,供试香菇菌株能适应液体培养环境,其适宜发酵培养温度为(25±1)℃,通气量1:0.5~1:1.摇瓶培养中48 h前为其调整期,48~144 h处于增殖生长期;从48 h起,pH值急剧下降,最终降至3.0左右趋于稳定,工业发酵培养96~120 h,生物量(鲜重)即达300g/L;提取的香菇营养液,氨基酸含量达18.52 g/L,多糖含量为14.75 g/L.

关 键 词:香菇  深层发酵  多糖  氨基酸
文章编号:1000-1565(2004)02-0172-03
修稿时间:2003-06-08

Studies on Submerged Fermentation Technology and Application of Lentinula edodes
WANG Qian,LIU Li-qun,YAN Lei-lei,JI Hong,CHEN Wen-jie. Studies on Submerged Fermentation Technology and Application of Lentinula edodes[J]. Journal of Hebei University (Natural Science Edition), 2004, 24(2): 172-174. DOI: 10.3969/j.issn.1000-1565.2004.02.015
Authors:WANG Qian  LIU Li-qun  YAN Lei-lei  JI Hong  CHEN Wen-jie
Affiliation:WANG Qian~1,LIU Li-qun~1,YAN Lei-lei~1,JI Hong~2,CHEN Wen-jie~2
Abstract:Study on the fermentation technology of Lentinula edodes shows that the subject can adapt to the submerged culture conditions. Its optimal fermentation temperature is (25±1)℃, ventilating quantity (1∶0.5)~1∶1. Its adjustment period and multiplication period are 0~48 h and 48~144 h respectively in shake flask culture. After 48 h, its pH goes down quickly, and comes to a stabilization when reaching pH 3.0. The biomass(fresh weight) reaches 300 g/L after 96~120 h's industrial fermentation culture. The content of amino acid and polysaccharide reaches 18.52 g/L and 14.75 g/L in its concentrated nutrient liquid.
Keywords:Lentinula edodes  submerged fermentation  polysaccharide  amino acid
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