|
|||||
|
|
Examination of cfiA-mediated carbapenem resistance in Bacteroides fragilis strains from a European antibiotic susceptibility survey |
|
| Authors: | Sóki J,Edwards R,Hedberg M,Fang H,Nagy E,Nord C E ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria |
| Affiliation: | aInstitute of Clinical Microbiology, Faculty of General Medicine, University of Szeged, H-6725 Szeged, Somogyi Béla tér 1, Hungary bDivision of Microbiology, University Hospital, Queen's Medical Centre, Nottingham, UK cDivision of Clinical Bacteriology, Department of Clinical Microbiology, Karolinska University Hospital, Karolinska Institute, Stockholm, Sweden dDepartment of Odontology, Division of Oral Microbiology, Umeå University, Sweden |
| Abstract: | Of 1284 Bacteroides strains collected in Europe in 2000 for antibiotic susceptibility surveillance, 65 isolates displayed imipenem minimum inhibitory concentrations (MICs) ≥1 mg/L and were chosen for a thorough analysis of their resistance mechanism. Twenty-five of the isolates were positive for the cfiA carbapenem resistance gene. The resistance rates were 0.8% and 1.3% for imipenem and meropenem, respectively. In six of the strains, insertion sequence (IS) elements (IS613, IS614B, IS1186 and IS1187) activated the cfiA gene. However, other strains displayed at least elevated carbapenem MICs or were carbapenem resistant and produced measurable carbapenemase activities but did not harbour IS elements in the region upstream of the cfiA gene. The major determinant of carbapenem resistance in Bacteroides fragilis is production of CfiA metallo-β-lactamase via activation of the cfiA gene by IS elements (higher level resistance) or by activation of its putative own promoter. |
| Keywords: | cfiA Bacteroides fragilis Imipenem Meropenem Insertion sequence (IS) elements |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |
