Inhibitory effect of a bioactivity-guided fraction from Rheum undulatum on the acid production of Streptococcus mutans biofilms at sub-MIC levels |
| |
Authors: | Kim Jeong-Eun Kim Hye-Jin Pandit Santosh Chang Kee-Wan Jeon Jae-Gyu |
| |
Affiliation: | Department of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 program, Chonbuk National University, Jeonju 561-756, Republic of Korea |
| |
Abstract: | Rheum undulatum root has been used traditionally in Korea for the treatment of dental diseases. The purpose of this study was to separate a fraction from R. undulatum showing anti-acid production activity against Streptococcus mutans biofilms and identify the main components in that fraction. Methanol extract of R. undulatum root and its fractions were prepared. To select a fraction exhibiting anti-acid production activity, suspension glycolytic pH-drop assay was performed. Among the fractions tested, dichloromethane fraction exhibited the strongest activity in a dose-dependent manner. To examine the effect of the selected fraction on the anti-acid production of S. mutans biofilms, 74 h old S. mutans biofilms were used. The selected fraction reduced the initial rate of acid production of S. mutans biofilms at sub-minimum inhibitory concentration (MIC) levels. HPLC qualitative analysis of the selected fraction indicated that the presence of anthraquinone derivatives, such as aloe-emodin, emodin, chrysophanol and physcion, as main components. |
| |
Keywords: | Rheum undulatum Streptococcus mutans Acid production Biofilms |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|