| 比较分析痰涂片及荧光定量PCR在诊断肺结核中的作用 |
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| 引用本文： | 杨上英.比较分析痰涂片及荧光定量PCR在诊断肺结核中的作用[J].中外医疗,2011,30(5):3-4. |
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| 作者姓名： | 杨上英 |
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| 作者单位： | 福建省福州市肺科医院检验科,福州,350104 |
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| 摘 要： | 目的探讨建立更快速、准确的痰结核分枝杆菌检测方法。方法采用痰涂片抗酸染色法和荧光定量PCR技术对痰结核分枝杆菌进行检。结果荧光定量PCR技术的阳性检出率是63.7%（571/897）,明显高于抗酸染色法的43.4%（389/897）。2种方法检测对照组的结果均为阴性,特异度为100%（103/103）。结论荧光定量PCR是一种快速、敏感性高、特异性强的结核分枝杆菌辅助诊断方法,具有重要的应用价值。
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| 关 键 词： | 结核分枝杆菌 抗酸染色 荧光定量PCR |
Comparative Analysis of Sputum Smear and Fluorescent Quantitative PCR in the Role of Diagnosis Tuberculosis |
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| YANG Shangying.Comparative Analysis of Sputum Smear and Fluorescent Quantitative PCR in the Role of Diagnosis Tuberculosis[J].China Foreign Medical Treatment,2011,30(5):3-4. |
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| Authors: | YANG Shangying |
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| Affiliation: | Yang Shangying Pulmonary Hospital,Fuzhou,Fujian 350104,China |
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| Abstract: | Objective To investigate the method for detection of Mycobacterium tuberculosis in sputum more quickly and exactly.Methods Sputum smear acid-fast stain and fluorescence quantitative PCR were used to detect Mycobacterium tuberculosis in sputum.Results the positive rates of fluorescence quantitative PCR was 63.7%（571/897）which was significantly higher than 43.4%（389/897）of acid-fast stain.The specificity of the control group was 100%（103/103）.The result of the two method was negative.Conclusion Fluorescence quantitative PCR is a rapid method for diagnosis of tuberculosis,which shows a high specificity and sensitivity.It is a useful tool for diagnosis of Mycobacterium tuberculosis in the future. |
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| Keywords: | Mycobacterium tuberculosis Acid-fast stain Fluorescence quantitative PCR |
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