参附注射液对戊巴比妥钠致心衰模型心肌细胞膜ATP酶和相关离子的影响

目的: 通过探讨参附注射液对新生大鼠心力衰竭心肌细胞Ca²⁺-ATP酶,Ca²⁺-Mg²⁺-ATP酶、Na⁺-K⁺-ATP酶活性及细胞内Na⁺,Mg²⁺,K⁺,Ca²⁺浓度的影响,揭示参附注射液强心作用的机制。 方法: 通过胰蛋白酶消化法和差速贴壁法获得心肌细胞,经0.8%戊巴比妥钠作用5min后,分别给予1.5, 3, 6 mg·L^-1(5,10,20 mL·L^-1)3个不同浓度的参附注射液,作用1 h,检测心肌细胞Ca²⁺-ATP酶,Ca²⁺-Mg²⁺-ATP酶,Na⁺-K⁺-ATP酶的活性和细胞内Na⁺,Mg²⁺,K⁺,Ca²⁺的浓度。 结果: 参附注射液能增加心衰模型心肌细胞的搏动强度,提高Ca²⁺-ATP酶和Ca²⁺-Mg²⁺-ATP酶的活性,抑制Na⁺-K⁺-ATP酶的活性,降低细胞内K⁺,Ca²⁺的浓度,升高细胞内Na⁺,Mg²⁺的浓度。 结论: 参附注射液对戊巴比妥钠致心衰细胞有明显的保护作用,且作用的发挥与调节心肌细胞Ca²⁺-ATP酶,Ca²⁺-Mg²⁺-ATP酶,Na⁺-K⁺-ATP酶的活性和细胞内Na⁺,Mg²⁺,K⁺,Ca²⁺的浓度有关。

Effects of the Shenfu Injection on ATPase and Related Ion of Heart Failure Model Myocardial Cell

Objective: To study the effects of Shenfu injection on the Ca²⁺-Mg²⁺-ATP ase, Ca²⁺-ATP ase, Na⁺-K⁺-ATP and Na⁺, Mg²⁺, K⁺, Ca²⁺ in the myocardial cell injured by nembutal. Method: The myocardial cell is gotten by trypsin digestion and purified by different velocity of adherence. After injured by 0.8% nembutal, the myocardial cell is administrated with 3 different concentrations of Shenfu Injection. Then mensurate the vitality of the Ca²⁺-Mg²⁺-ATP ase, Na⁺-K⁺-ATP ase and Ca²⁺-ATP ase and Na⁺, Mg²⁺, K⁺, Ca²⁺ in the cell 1 h later. Result: The Shenfu injection can increase the strength of the myocardial cell beating injured by nembutal, and enhance the activity of Ca²⁺-ATP ase and the Ca²⁺-Mg²⁺-ATP ase, inhibit the the activity of Na⁺-K⁺-ATP, enzyme increase the content of Na⁺, Mg²⁺ and decrease the content of K⁺, Ca²⁺ in the myocardial cell. Conclusion: The Shenfu injection can protect the myocardial cell injured by nembutal, and the function is relevent to the effects of Shenfu injection on the Ca²⁺-Mg²⁺-ATP ase, Na⁺-K⁺-ATP ase, Ca²⁺-ATP ase and the content of Na⁺, Mg²⁺, K⁺, Ca²⁺ of myocardial cell.