喇曼光谱对血糖的半定量分析

血糖检测一般采用酶化和生化方法,但这些方法都是有损破坏性的检测方法.本文利用喇曼光谱技术来检测血糖浓度,并且建一种新的数据分析方法来分析血糖的含量,以探索一种无损、快速的血糖检测方法.以小白鼠为实验模型,麻醉的小白鼠在注射葡萄糖后半个小时开始抽取小鼠尾巴处的血液进行喇曼光谱的获取,此后每间隔15 min对小鼠尾巴进行抽血并获取血液的喇曼光谱,在每次测量小鼠血液喇曼光谱的同时用血糖仪来监测血糖浓度的变化情况以用来做参比.1 125 cm^-1是葡萄糖的喇曼特征峰,血液中的葡萄糖称为血糖,因此我们把血液光谱中的1 125 cm^-1作为血糖峰,1 549 cm^-1为血红蛋白的喇曼特征峰,人体中的血红蛋白是稳定的,所以本文以血红蛋白的峰1 549 cm^-1作为内标来研究血液喇曼光谱中血糖峰的强度.结果表明1 125 cm^-1/1 549 cm^-1的变化可以很好地与血糖变化相对应,并且具有良好的线性关系.利用喇曼光谱技术可以无损地对血糖进行半定量分析.

Semi-quantitative Analysis of Blood Glucose Using Raman Spectroscopy

The measurement of blood glucose are enzymatic and biochemical methods which are destructive. Raman technique is used to detect blood glucose and a new data analysis method is established to analyse blood glucose concentration, which is a noninvasive and rapid blood glucose tests. Using mice as experimental models, Raman spectra of blood taken from the tails are acquried half an hour after injection of glucose. Raman spectra are collected every 15 minutes and accompanied by blood glucose meter to get the blood glucose value to be reference. 1 125 cm^-1 is characteristic peak of glucose and glucose in blood is called blood glucose, so 1 125 cm^-1 is chosen as the peak of blood glucose in the Raman spectra of blood. 1 549 cm^-1 is characteristic peak of hemoglobin which is stable in the body, so 1 549 cm^-1is used to be internal standard to research 1 125 cm^-1 intensity. The result shows that the change of 1 125 cm^-1/1 549 cm^-1 may well correspond with changes in blood glucose and they have a good linear relationship. The experiments show that this Raman technique can reflect the changes of blood sugar levels noninvasive.