转录因子Slug、E-钙黏蛋白、N-钙黏蛋白在胃肠道间质瘤中的表达及其意义

目的 探讨转录因子Slug、E-钙黏蛋白、N-钙黏蛋白在胃肠道间质瘤(GIST)中的表达及其临床意义.方法 收集2004年5月至2007年10月于中南大学湘雅医院行手术切除的胃肠间质瘤标本78例,患者年龄28～87岁,男48例,女30例.采用免疫组织化学技术检测GIST组织中Slug、E-钙黏蛋白、N-钙黏蛋白的表达情况,并分析其与GIST临床病理特征之间的关系.结果 Slug、E-钙黏蛋白、N-钙黏蛋白在78例GIST组织中的阳性表达分别为42例(53.8％),28例(35.9％)和59例(75.6％),差异有统计学意义(x2 =24.98,P＜0.05).Slug在发生远处转移、侵犯或转移的GIST组织中的表达率明显高于未发生远处转移、未侵犯或转移的GIST组织75.0％ (18/24)比44.4％( 24/54),63.6％ (28/44)比41.2％ (14/34),均P＜0.05],E-钙黏蛋白在发生远处转移的GIST组织中的表达率明显低于未发生远处转移的GIST组织16.7％ (4/24)比44.4％( 24/54),P＜0.05],N-钙黏蛋白在各项临床病理特征间的表达差异均无统计学意义(均P＜0.05).Slug与E-钙黏蛋白在GIST中的表达呈负相关(rs=-0.267,P=0.018),与N-钙黏蛋白的表达无明显关联(rs=0.056,P=0.625).结论 在远处转移的GIST组织中Slug的表达率明显高,而E-钙黏蛋白的表达率则明显低,二者的表达呈负相关,与GIST的转移有密切关系.

Expression and significance of Slug, E-cadherin and N-cadherin in gastrointestinal stromal tumors

Objective To explore the expression and clinical significance of Slug,E-cadherin and N-cadherin in gastrointestinal stromal tumors (GIST).Methods Seventy eight GIST specimens removed surgically from 2004 to 2007 were collected from the Department of Gastrointestinal Surgery at Guizhou Provincial People's Hospital.There were 48 males and 30 females with an age range of 28 -87 years old.The expressions of Slug,E-cadherin and N-cadherin in GIST were determined by immunohistochemistry.And the correlations with their clinicopathologic characteristics were analyzed.Results The positive rates of Slug,E-cadherin and N-cadherin in GIST were 53.8％ (42/78),35.9％ (28/78) and 75.6％ (59/78) respectively.And the differences were statistically significant (x2 =24.98,P ＜ 0.05 ).Slug was expressed markedly higher in the cases of GIST with distant metastasis or distant metastasis and local invasion: 75％ (18/24) vs 44.4％ (24/54),63.6％ (28/44) vs41.2％ (14/34),both P＜0.05.And E-cadherin was expressed markedly lower in the cases of GIST with distant metastasis: 16.7％ (4/24) vs 44.4％ (24/54), P ＜ 0.05. The expression of N-cadherin was not significantly different between its clinicopathological characteristics ( allP ＞0.05 ).The expression of Slug correlated negatively with that of Ecadherin (rs =-0.267,P =0.018).But it had no correlation with that of N-cadherin (rs =0.056,P =0.625).Conclusion Slug is expressed markedly higher while E-cadherin markedly lower in metastatic GIST,and both are closely correlated with the metastasis of GIST.