非小细胞肺癌患者外周血CD4＋CD25＋Foxp3＋调节性T细胞检测及临床意义

目的]探讨非小细胞肺癌患者外周血CD4＋CD25＋Foxp3＋调节性T细胞（Treg）表达及临床意义。方法]流式细胞术检测30例非小细胞肺癌组患者及12例健康志愿者外周血CD4＋CD25＋Foxp3＋Treg百分比。结果]肺癌组外周血CD4＋CD25＋Foxp3＋细胞百分比（6.24%±2.01%）高于健康对照组（4.83%±0.85%）（P〈0.05）,外周血CD4＋CD25＋Foxp3＋细胞百分比在肺癌患者不同性别、年龄、病理、KPS及肿瘤标志物正常与否之间比较无差异（P〉0.05）,Ⅳ期患者表达高于Ⅰ～Ⅲ期患者（P〈0.05）,带瘤患者表达高于无瘤患者（P〈0.05）,有气虚证、血瘀证患者高于无气虚证、血瘀证患者（P〈0.05）。结论]检测肺癌患者外周血CD4＋CD25＋Foxp3＋Treg表达在了解患者肿瘤进展情况和机体免疫功能状态方面有一定价值,其表达升高与患者出现气虚证和血瘀证有关。

Peripheral CD4+CD25+Foxp3+Regulatory T cells in Patients with NSCLC and Its Clinical Significance

Purpose] To investigate the expression of CD4＋CD25＋Foxp3＋ regulatory T cells（Treg）in peripheral blood in patients with NSCLC and its significance.Methods] The proportion of CD4＋CD25＋Foxp3＋ Treg in thirty cases with NSCLC and 12 healthy controls were measured by flow cytometry.Results] The proportion of CD4＋CD25＋Foxp3＋ Treg in peripheral blood from patients with NSCLC（6.24%±2.01%） was higher than that from healthy controls（4.83%±0.85%）（P0.05）.The proportion of peripheral CD4＋CD25＋Foxp3＋ Treg in patients with NSCLC was not different with agender,age,pathology,KPS score and tumor marker level（P0.05）.The proportion of CD4＋CD25＋Foxp3＋ Treg in the patients with NSCLC stage Ⅳ was higher than that in stage Ⅰ～Ⅲ（P0.05）.The proportion of CD4＋CD25＋Foxp3＋ Treg in the patients bearing tumor was higher than those without tumor（P0.05）.The proportion of CD4＋CD25＋Foxp3＋ Treg in patients with Qi deficiency syndrome or blood stasis syndrome was higher than those without the syndromes（P0.05）.Conclusion] Detecting the expression of CD4＋CD25＋Foxp3＋ Treg in peripheral blood of patients with NSCLC is helpful for knowing cancer progression and the patients immunity.The increase of the proportion of CD4＋CD25＋Foxp3＋ Treg closely correlates to Qi deficiency syndrome and blood stasis syndrome.