Naringin Protects against Rotenone-induced Apoptosis in Human Neuroblastoma SH-SY5Y Cells |
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| Authors: | Hak-Jae Kim Jeong Yoon Song Hae Jeong Park Hyun-Kyung Park Dong Hwan Yun and Joo-Ho Chung |
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| Affiliation: | 1Department of Pharmacology and Kohwang Medical Research Institute, School of Medicine, Kyung Hee University, Seoul 130-701, Korea.;2East-West Neo Medical Center, School of Medicine, Kyung Hee University, Seoul 130-701, Korea.;3Department of Physical Medicine and Rehabilitation, School of Medicine, Kyung Hee University, Seoul 130-701, Korea. |
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| Abstract: | Rotenone, a mitochondrial complex I inhibitor, can induce the pathological features of Parkinson''s disease (PD). In the present study, naringin, a grapefruit flavonoid, inhibited rotenone-induced cell death in human neuroblastoma SH-SY5Y cells. We assessed cell death and apoptosis by measuring mitogen-activated protein kinase (MAPKs) and caspase (CASPs) activities and by performing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Naringin also blocked rotenone-induced phosphorylation of Jun NH2-terminal protein kinase (JNK) and P38, and prevented changes in B-cell CLL/lymphoma 2 (BCL2) and BCL2-associated X protein (BAX) expression levels. In addition, naringin reduced the enzyme activity of caspase 3 and cleavages of caspase 9, poly (ADP-ribose) polymerase (PARP), and caspase 3. These results suggest that naringin has a neuroprotective effect on rotenone-induced cell death in human neuroblastoma SH-SY5Y cells. |
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| Keywords: | Apoptosis Naringin Parkinson''s disease Rotenone SH-SY5Y |
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