| 基于石墨烯-海藻酸钠复合材料的辣根过氧化物酶传感器研究 |
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| 引用本文： | 肖福兵,杨,欣,田,锐,张瑞莲,梅琼之,吴朝阳.基于石墨烯-海藻酸钠复合材料的辣根过氧化物酶传感器研究[J].化学传感器,2014(1):57-63. |
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| 作者姓名： | 肖福兵 杨 欣 田 锐 张瑞莲 梅琼之 吴朝阳 |
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| 作者单位： | [1]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,湖南长沙410082 [2]怀化学院化学与化学工程系,湖南怀化418008 |
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| 基金项目： | 基金项目：国家自然科学基金（批准号：J1210040,J1103312） |
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| 摘 要： | 构建了一种以海藻酸钠-石墨烯(SA-GR)为基底的新型过氧化氢电化学酶传感器。利用滴涂法将生物相容性良好的海藻酸钠-石墨烯复合物固定在玻碳电极表面,再通过酰胺键将HRP连接在SA-GR复合膜上,从而制备出了性能良好的过氧化氢电化学酶传感器。该传感器重现性好、灵敏度较高,并且响应速度快(3 s),米氏常数较低(Km=0.663),对H2O2检测的线性范围为1.0×10-4～1.2×10-3mol/L,检测下限为5.7×10-6mol/L。
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| 关 键 词： | 海藻酸钠 石墨烯 辣根过氧化物酶 |
Graphene-sodium alginate composite based horseradish peroxidase biosensor |
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| Xiao Fu-bing,Yang Xin,Tian Rui,Zhang Rui-lian,Mei Qiong-zhi,Wu Zhao-yang.Graphene-sodium alginate composite based horseradish peroxidase biosensor[J].Chemical Sensors,2014(1):57-63. |
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| Authors: | Xiao Fu-bing Yang Xin Tian Rui Zhang Rui-lian Mei Qiong-zhi Wu Zhao-yang |
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| Affiliation: | 1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China) (2. Department of Chemistry and Chemical Engineering, Huaihua Collge, Huaihua 418008, China) |
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| Abstract: | A new type of enzyme electrochemical sensor for hydrogen peroxide was constructed alginate-graphene （SA-GR） composite. The biocompatible alginate-graphene composite is fixed to the glassy carbon electrode surface by drop-coating method, and then HRP is linked to the SA-GR membrane through an amide bond, thereby the HRP electrochemical biosensor with good performance is prepared for hydrogen peroxide. This biosensor has high sensi- tivity, good reproducibility, fast response （3 s）, low Miehaelis constant （Km=0.663） and a good linear relationship for detection H202 in the range from 1.0×10^-4 to 1.2×10^-3 mol/L. The detection limit was estimated to be 5.7×10^-6mol/L. |
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| Keywords: | sodium alginate graphene horseradish peroxidase |
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