跳骨片中马兜铃酸A的HPLC限量检查

目的建立测定跳骨片中马兜铃酸A的高效液相色谱的分析方法。方：法采用RP-HPLC法测定。用NovapakC18色谱柱（250mm×4．6mm，5岬），以已腈一甲醇-1％冰醋酸溶液（28：28：44）为流动相，流速为lmL·min-1，检测波长390rim，柱温为宣温。进样量为5“k结果马兜铃醢A的保留时问约为14．7min，与其他峰的分离度大干1．5。马兜铃酸A的线性范围为0．49869ug·mL-1～3．49083ug·mL-1，y=0．9998，最低检测限为4．128×10_。pg（信噪比为5：1），平均回收率为97．89％（n=5）。结论＂ig7Y：,-k简便快速．灵敏度高，结果准确可靠，可用于跳骨片中马兜铃酸A的含量测定，跳骨片中马兜铃酸A未检出。

Limit test of aristolochic acid A in Tiaogu tablets by HPLC

OBJECTIVE To establish a method for the determination of aristolochie acid A in Tiaogu tablets by HPLC. METHODS We used a RPHPLC method with Novapak Cls column（5ttm, 4.6mm x 250 ram）, and the mobile phase was themixture of acetonitrilemethanolwater（28 ： 28 ： 44）, and the flow rate was 1.0mL· min- 1 . with the detection wavelength of 390nm. oven temperature：room temperature. Injection volume：5uL. RESULTS The retention time of aristolochie acid A was 14.7rain, resolution〉l. 5. The linear range of aristolochic acid A was 0.49869/_tg. mL- 1 - 3. 49083ug· mL-1, r = 0. 9998, detectable limitation of 4. 128 x 10 - 3ug （signal to noise ratio is 5 ： 1）, the average recovery was 97.89 %, （n = 6）. CONCLUSION The method is simple, rapid, sensitive,and the result is accurate, it can be used for determination of aristolochic acid A in Tiaogu tablets. Resultes aris- tolochic acid A was not detected in Tiaogu tablets.