Determination of bixin and norbixin in meat using liquid chromatography and photodiode array detection |
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| Authors: | H Noppe S Abuín Martinez K Verheyden J Van Loco R Companyó Beltran |
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| Affiliation: | 1. Faculty of Veterinary Medicine, Laboratory of Chemical Analysis , Research Group of Veterinary Public Health and Zoonoses, Ghent University , Salisburylaan 133, B-9820 Merelbeke, Belgium;2. Faculty of Chemistry, Department of Analytical Chemistry , Barcelona University , Avinguda Diagonal, 647, E-08028 Barcelona, Spain;3. Department of Pharmaco-bromatology, Food Section , Institute of Public Health , J. Wytsmanstraat, B–1050 Brussels, Belgium |
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| Abstract: | The development of an analytical method that enables routine analysis of annatto dye, specifically bixin and norbixin, in meat tissue is described. Liquid-solid extraction was carried out using acetonitrile. Analysis was by HPLC with photodiode array detection using two fixed wavelengths (458 and 486 nm). The possibilities of ion trap mass spectrometry (MS) were also assessed. Method performance characteristics, according to Commission Decision 2002/657/EC, were determined, with recoveries between 99 and 102% and calibration curves being linear in the 0.5–10 mg kg?1 range. The limit of quantification was 0.5 mg kg?1. |
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| Keywords: | annatto bixin norbixin meat liquid chromatography photodiode array |
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