| 罗格列酮对小鼠Ⅱ型葡萄糖载体mRNA表达的影响 |
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| 引用本文： | 李天洙,李霞,张学武.罗格列酮对小鼠Ⅱ型葡萄糖载体mRNA表达的影响[J].延边医学院学报,2007,30(3):164-166. |
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| 作者姓名： | 李天洙 李霞 张学武 |
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| 作者单位： | 延边大学基础医学院生物化学与分子生物学教研室 吉林延吉133000 |
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| 摘 要： | 目的]探讨罗格列酮对小鼠Ⅱ型葡萄糖载体(mGLUT2)mRNA表达的影响.方法]采用分子克隆技术将过氧化物酶体增殖物受体γ(PPARγ)、维甲酸类受体X(RXRα)及mGLUT2cDNA分别克隆到表达载体pCMX和pGL3b上.PPARγ,RXRα,mGLUT2克隆载体转染NIH3T3细胞,处理或不处理罗格列酮,应用荧光素酶活性测定法及RNA印迹方法等测定罗格列酮对mGLUT2重组体荧光素酶活性调节及对mRNA表达的影响.结果]罗格列酮可激活mGLUT2重组体荧光素酶的活性,亦可增加mRNA的表达水平.结论]罗格列酮可增强mGLUT2的表达,可能参与PPARγ对mGLUT2的调节过程.
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| 关 键 词： | 葡萄糖转运蛋白质类 易化性 RNA 信使 罗格列酮 |
| 文章编号： | 1000-1824（2007）03-0164-03 |
| 收稿时间： | 2007-04-12 |
| 修稿时间： | 2007年4月12日 |
Effect of the Rosiglitazone on mouse glucose transporter Ⅱ mRNA expression |
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| LI Tian-zhu,LI Xia,ZHANG Xue-wu.Effect of the Rosiglitazone on mouse glucose transporter Ⅱ mRNA expression[J].Journal of Medical Science Yanbian University,2007,30(3):164-166. |
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| Authors: | LI Tian-zhu LI Xia ZHANG Xue-wu |
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| Affiliation: | Department of Biochemistry and Molecular Biology, Yanbian University College of Basic Medicine, Yanji 133000, Jilin, China |
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| Abstract: | OBJECTIVE To understand the possibility of effect of the Rosiglitazone on mouse glucose transporter Ⅱ(mGLUT 2) mRNA expression.METHODS PPARγ,RXRα and mGLUT 2 cDNA clone into pCMX and pGL3 b expression vector.The recombinant DNA transfected into NIH 3 T 3 cells,treated the rosigiltazone and confirm the mGLUT 2 activity and mRNA expression by the Rosiglitazone through PPARγ activation with luciferase assay and Northern blot.RESULTS The Rosigiltazone activates the mGLUT 2 activity and increase the mGLUT 2 mRNA expression through PPARγ activation.CONCLUSION The Rosigiltazone perhaps binds to PPARγ and regulations mGLUT 2 mRNA expression. |
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| Keywords: | glucose transport proteins facilitative RNA messenger rosigiltazone |
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