血管生成素和VEGF在人肝细胞癌血管生成作用机制的研究

目的探讨血管生成素(Ang)及受体Tie-2、血管内皮牛长因子(VEGF)在肝细胞癌血管生成和进展中的作用。方法用RT-PCR和免疫组织化学方法分析28例肝细胞痛(HCC)、10例肝硬化和10例正常肝标本。观察血管生成素及受体、VEGF的表达与HCC肿瘤血管生成和临床病理的关系。结果Ang／Tie-2，VEGF在肝细胞癌表达明显上调，免疫组织化学也显示Ang-2和VEGF，Tie-2蛋白在肝癌表达增强。Ang-2／Ang-1 mRNA比值、VEGF mRNA与肿瘤血管侵犯及CD34染色的微血管密度相关。结论Ang／Tie-2，VEGF、在HCC肿瘤血管生成和进展中起重要作用。

Effects of angiopioetins/Tie-2 and VEGF expression on hepatocellular carcinoma angiogenesis

Objective To investigate the effects of angiopioetins and tyrosine kinase receptor Tie-2 ,vascular endothelial growth factor (VEGF)on the angiogenesis and progression of hepatocellular carcinoma(HCC). Methods With the methods of RT-PCR and immunohistochemistry, the specimens from 28 HCC patients, 10 cirrhotics, and 10 normal livers were analysed, and the relationship between angiopioetins, VEGF (expression) and the clinical pathological characteristics of HCC was studied. Results Ang/Tie-2 and VEGF were significantly up-regulated in HCC compared to cirrhotic tissue and normal liver tissue. (Immunohistochemical) staining also showed increased expression of Ang-2,VEGF,Tie-2 protein in HCC. A high Ang-2/Ang-1mRNA ratio and high VEGF in HCC were closely associated with tumor vascular invasion and microvascular density level which assesssed by CD34. Conclusions Ang /Tie-2 and VEGF may play critical roles in promoting tumor angiogenesis and progression in human HCC.