高迁移率族蛋白B1、基质金属蛋白酶-9和血管内皮生长因子-C在甲状腺乳头状癌中的表达及意义

目的研究甲状腺乳头状癌（papillary thyroid carcinoma，PTC）中高迁移率族蛋白B1（high mobility group box1，HMGB1）、基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）和血管内皮生长因子-C（vascular endothelial growth factor-C，VEGF—C）的表达及与PTC临床病理特征的关系。方法应用免疫组化SP法和原位杂交技术联合检测58例PTC、20例甲状腺腺瘤、25例结节性甲状腺肿和10例正常甲状腺组织HMGB1、MMP-9、VEGF—C蛋白和HMGB1 mRNA的表达。结果HMGB1、MMP-9、VEGF-C蛋白和HMGB1 mRNA在PTC中的表达显著高于3种非癌组织（P〈0．05）。HMGB1、MMP-9蛋白和HMGB1 mRNA表达的阳性率与肿瘤的直径及淋巴结转移密切相关（P〈0．05）。VEGF-C蛋白表达的阳性率与淋巴结转移密切相关（P〈0．05）。HMGB1、MMP-9、VEGF—C蛋白和HMGB1 mRNA表达的阳性率与患者的性别、年龄无关（P〉0．05）。HMGB1、MMP-9和VEGF—C蛋白的表达均呈两两正相关（P〈0．05），HMGB1蛋白与HMGB1 mRNA的表达也呈正相关（P〈0．05）。结论HMGB1、MMP-9、VEGF—C蛋白和HMGB1 mRNA的表达与PTC的发生发展及浸润、转移有关，检测其表达对判断临床进展及推测预后有一定参考价值。

Expression of HMGB1, MMP-9 and VEGF-C in papillary thyroid carcinoma

Objective To study the expression of high mobility group box 1 （ HMGB1 ）, matrix metalloproteinase-9（MMP-9） and vascular endothelial growth factor-C （VEGF-C）gene in papillary thyroid carcinoma （PTC） and their significance. Methods Expression of HMGB1, MMP-9, VEGF-C proteins and HMGB1 mRNA was detected by technology of immunohistochemistry（SP） and in situ hybridization in 58 cases of PTC tissues, 20 cases of thyroid adenoma tissues, 25 cases of nodular goiter tissues and 10 cases of normal thyroid tissues. Re- suits Expression of HMGB1, MMP-9, VEGF-C proteins and HMGB1 mRNA in PTC tissues was much higher than that in the 3 non-cancerous tissues （P 〈 0. 05）. The positive expression rate of HMGB1, MMP-9 proteins and HMGB1 mRNA was closely associated with the thyroid carcinoma size and lymph node metastasis （P 〈 0. 05 ）. There was also closely association between the positive expression rate of VEGF-C protein and lymph node metastasis（P 〈 0. 05）. The positive expression rate of HMGB1, MMP-9, VEGF-C proteins and HMGB1 mRNA had no association with patients＇ sex or age （P 〉 0. 05 ）. The expression of HMGB1, MMP-9 and VEGF-C proteins had positive correlation with each other（P 〈 0. 05）. The expression of HMGB1 protein and HMGB1 mRNA was also positively correlated with each other（P 〈 0. 05）. Conclusion Expression of HMGB1, MMP-9, VEGF- C proteins and HMGB1 mRNA in PTC is correlated with the progress,invasion, metastasis of PTC, by detecting them may help to predict the clinical progress and prognosis.