| 固相萃取和高效液相色谱法测定甜茶中的黄酮类物质 |
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| 引用本文： | 杨亚玲,赵红芳,苏毅,赵榆林,周强. 固相萃取和高效液相色谱法测定甜茶中的黄酮类物质[J]. 食品科学, 2006, 27(9): 197-199 |
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| 作者姓名： | 杨亚玲 赵红芳 苏毅 赵榆林 周强 |
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| 作者单位： | 昆明理工大学生物与化学工程学院 |
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| 摘 要： | 研究了用固相萃取预分离,高效液相色谱法测定甜茶中黄酮类物质的方法。甜茶中的黄酮用80%乙醇溶液回流提取,提取液用WatersSep-Pak-C18固相萃取小柱预分离脱脂,以WatersNova-Pak-C18(3.9×150mm,5μm)色谱柱为固定相,以0.05mol/L磷酸二氢钾缓冲溶液和甲醇的比例为40:60(V/V)为流动相,在该色谱条件下,甜茶中主要的黄酮成分均达到基线分离;用紫外二极管矩阵检测器在360nm波长处检测,并作了色谱峰纯度分辨。方法标准回收率为97%～103%,相对标准偏差为0.87%～2.2%。
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| 关 键 词： | 高效液相色谱 固相萃取 甜茶 黄酮 |
| 文章编号： | 1002-6630（2006）09-0197-04 |
| 收稿时间： | 2005-09-28 |
| 修稿时间： | 2005-09-28 |
Study on Flavonoid Assay in Lithhocarpus polgstachrch Rehd with Solid Phase Extraction and HPLC |
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| YANG Ya-ling,ZHAO Hong-fang,SU Yi,ZHAO Yu-lin,ZHOU Qiang. Study on Flavonoid Assay in Lithhocarpus polgstachrch Rehd with Solid Phase Extraction and HPLC[J]. Food Science, 2006, 27(9): 197-199 |
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| Authors: | YANG Ya-ling ZHAO Hong-fang SU Yi ZHAO Yu-lin ZHOU Qiang |
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| Affiliation: | Faculty of Biologic and Chemical Engineering, Kunming University Science of Technology, Kunming 650224, China |
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| Abstract: | A high performance liquid chromatography (HPLC) method for the flavonoid assay in Lithhocarpus polgstachrch Rehd was studied. The flavonoid was extracted from the sample with 80% of ethanol by heated reflux. Then the extract was degreased by solid phase extraction with Sep-Pak-C18 cartridge. The flavonoid was separated on a Nova-Pak-C18 chromatogram column (3.9×150mm, 5μm), with methanol and 0.05mol/L potassium dihydrogen phosphate buffer solution as mobile phase at 60:40 (V/V), and monitored with the photodiode array detector at 360nm. The recoveries of the flavonoid are 97%～103%, and relative standard deviations are 0.87%～2.2%. |
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| Keywords: | HPLC solid phase extraction Lithhocarpus polgstachrch rehd flavonoid |
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