胃癌组织中NF-κB和COX-2的表达及临床意义

目的：探讨核因子-κB（nuclear factor-kappa B，NF-κB）和环氧合酶-2(cyclooxygenase-2，COX-2)的表达与胃癌发生发展的关系，为开展以NF-κB和COX-2为靶标的胃癌防治提供理论依据。方法：采用免疫组织化学染色法（SP法）检测对照组10例正常胃黏膜及实验组50例胃癌组织中NF-κB和COX-2的表达情况，分析它们在不同组织中的表达、相互关系及与胃癌的关系。结果：实验组NF-κB和COX-2的表达阳性率明显高于对照组（P<0.001）；NF-κB表达与胃癌淋巴结转移、分化程度有关（P<0.05），COX-2 表达与淋巴结转移有关（P<0.05）；胃癌组织中NF-κB与COX-2的表达呈正相关关系，相关系数r=0.5238 （P<0.01）。结论：NF-κB和COX-2参与胃癌的形成，且与胃癌的侵袭、转移及预后有一定的关系。NF-κB与COX-2表达呈正相关，提示它们在胃癌的发生发展中起协同作用。

Expression and clinical significance of NF-KB and COX-2 in gastric carcinomas

ObjectiveTo detect the expression of nuclear factor-kappa B (NF-κB) and cyclooxygenase-2 (COX-2) in the tissue of gastric cancer， and to discuss the correlation of NF-κB and COX-2 expression.MethodsThe formalin-fixed and paraffin-embedded tissues from 50 cases of gastric carcinoma, 10 cases of normal tissues were evaluated for the expression of NF-κB and COX-2 by SP immunohistochemistry, the levels of these proteins in tissues with different types and their correlation with gastric carcinoma were analyzed. ResultsThe positive rate of NF-κB and COX-2 in gastric carcinoma was significantly higher than that in normal tissues (P<0.001).The expression of NF-κB was associated with lymph node metastasis and differentiation (P<0.05);The expression of COX-2 was associated with lymph node metastasis (P<0.05). NF-κB expression was positive correlated with COX-2 in gastric carcinoma, coefficient correlation was 0.5238 (P<0.01). ConclusionNF-κB and COX-2 play an important part in carcinogenesis, and are involved in the formation of gastric carcinoma. Besides, it is also associated with the invasion, metastasis and prognosis of gastric carcinoma. NF-κB expression is positive correlated with COX-2 in gastric carcinoma and has synergistic effect on the carcinogenesis and development of gastric carcinoma.