肺抑瘤合剂对A549裸鼠移植瘤生长抑制的实验研究

目的:探讨肺抑瘤合剂对肺癌A549细胞系裸鼠皮下移植瘤生长的抑制作用并探讨其机制。方法:建立肺癌A549细胞系裸鼠移植瘤动物模型,随机分为对照组、肺抑瘤合剂组及顺铂组灌胃或腹腔注射给药干预,观察肺抑瘤合剂对裸鼠移植瘤生长的影响;采用Real-Time PCR方法检测表皮生长因子受体(EGFR)表达,Western-Blot方法检测EGFR、P38、GSK3β和AKT表达。结果:肺抑瘤合剂有较为明显的抑瘤效果(P0.05)。与对照组相比,肺抑瘤合剂组和顺铂组移植瘤组织中EGFR m RNA表达水平明显下调(P0.05)。EGFR及p-EGFR蛋白水平有下降趋势,但无统计学意义。P38、GSK3β及AKT蛋白表达无明显变化,而p-P38、p-GSK3β及p-AKT的水平明显下调(P0.05)。结论:肺抑瘤合剂对肺癌A549裸鼠移植瘤有明显抑制作用,可能通过抑制P38、GSK3β和AKT蛋白的磷酸化水平,起到抑制移植瘤生长的效果。

Experimental Study of Feiyiliu Mixture on Inhibiting the Growth of Lung Cancer A549 Xenograft Tumor in Nude Mice

Objectives:To observe the effect of Feiyiliu mixture on inhibiting lung cancer A549 xenograft tumor innude mice and to investigate the underlying mechanisms. Method:Nude micewere transplanted with lung cancer A549 cell line,and weredivided into the control group,Feiyiliu mixture group,and cisplatin group. The effect of Feiyiliu mixture on inhibiting the growth of xenograft tumor was observed. Epidermal growth factor receptor(EGFR) m RNA levelwas detected by real-time PCR,and EGFR,P38,GSK3β and AKT levels were detected withwestern blot assay. Results:Feiyiliu mixture had significant effects of inhibiting tu mor growth(P<0.05).Compared with the control group,EGFR m RNA level in the tumor tissues in both the Feiyiliu mixture group and cisplatin group was down-regulated(P<0.05),and the protein level of EGFR and p-EGFR also showed a trend of decreasing,but without statistical significance;protein level of P38,GSK3βand AKT showed no significant changes,but the protein levels of p-P38,p-GSK3β and p-AKTwere significantly down-regulated(P<0.05). Conclusion :Feiyiliu mixture couldinhibit the growth of lung cancer A549 xenograft tumor in nude mice,possibly through inhibiting phosphorylation levels of protein P38,GSK3β and AKT to restrain tumor growth.