血小板衍生生长因子受体在膀胱癌中的表达意义

目的　探讨血小板衍生生长因子受体 (PDGFR)表达与膀胱移行上皮细胞癌发生、生物学行为及预后的关系 .方法　采用免疫组织化学技术——两步法检测 6 8例膀胱移行细胞癌和 10例正常膀胱组织 PDGFR表达及膀胱癌组织中微血管密度 (MVD) .结果　 PDGFR阳性染色分布于癌细胞、间质、血管及炎症细胞的胞质及胞膜 .膀胱癌组织PDGFR阳性率为 83.8% ,明显高于正常膀胱组织 (30 .0 % ,P<0 .0 1) ,膀胱癌组织中 PDGFR阳性表达组的微血管密度(MVD)明显高于 PDGFR阴性表达组 (85± 30 vs 36± 15 ,P<0 .0 1) .结论　 PDGFR表达与正常膀胱组织生长有关 ,过度表达与癌的形成、血管的形成及炎症细胞功能相关 ,未发现PDGFR表达与肿瘤分期、分级及预后相关 .

Expression of platelet-derived growth factor receptor in human bladder carcinoma

AIM　To study the relationship between expression of platelet-derived growth factor receptor(PDGFR) and the genesis, biological behavior and prognosis of bladder transitional cell carcinoma. METHODS　Expression of PDGFR and microvessel density（MVD） were investigated in 68 cases of transitional cell carcinoma in bladder and 10 cases of normal bladder tissues with immunohistochemical technique according to the procedures of classical two-steps. RESULTS　It was revealed that the PDGFR positive staining rate in tumor tissues(83.8%, 57/68) was significantly higher (P<0.01) than that in normal tissues(30%, 3/10)； the positive staining was confined to the cytoplasms and membranes of epithelial, interstitial, vascular smooth muscle and inflammatory cells. MVD was higher in the positive expression of PDGFR than in the negative patients(85±30 vs 36±15, P<0.01). CONCLUSION　Expression of PDGFR is related to the growth of the normal bladder tissues, but the increased expression of PDGFR is associated with malignant transformation and development of bladder carcinoma, angiogenesis in the bladder cancer and the function of inflammatory cells. There might be no significant correlation between expression of PDGFR and tumor grade, stage and prognosis.