醋炙降低芫花二氯甲烷部位对大鼠肝肾细胞毒性的分析

目的:比较醋炙前后芫花二氯甲烷提取物对于大鼠正常肝细胞BRL及大鼠正常肾细胞NRK的细胞毒性变化。方法:选择BRL及NRK细胞为研究对象,采用噻唑蓝(MTT)比色法评价生、醋芫花二氯甲烷部位对BRL与NRK活性的影响;测定NRK细胞中尿素氮(BUN),乳酸脱氢酶(LDH),谷胱甘肽(GSH)的含量,以及BRL细胞中丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),碱性磷酸酶(ALP),LDH,GSH的含量,用于评价生、醋芫花二氯甲烷部位对BRL和NRK的氧化损伤作用。结果:与空白组相比,生芫花二氯甲烷部位对NRK和BRL细胞具有显著毒性,显著提高NRK细胞中BUN和LDH的含量(P 0. 05,P 0. 01);显著提高BRL细胞中AST,ALT,ALP和LDH的含量(P 0. 05,P 0. 01);降低NRK和BRL细胞中的GSH含量。与生芫花二氯甲烷部位相应剂量组相比,醋芫花二氯甲烷部位相应剂量组能提高NRK和BRL的细胞活性,降低NRK细胞中BUN和LDH的含量,降低BRL细胞中ALT,AST,ALP和LDH的含量;提高NRK和BRL细胞中GSH的含量。结论:醋炙可降低芫花二氯甲烷部位对大鼠肝肾细胞的毒性,提高大鼠肝肾细胞功能与抗氧化能力。

Effect of Processing with Vinegar on Hepatorenal Toxicity of Dichloromethane Site of Genkwa Flos

Objective:To campare the hepatotoxicity on BRL and nephrotoxicity on NRK caused by dichloromethane site of Genkwa Flos before and after being processed with vinegar. Method:BRL of normal hepatocytes and NRK of normal renal cells in rats were selected as the subjects.Thiazolyl blue tetrazolium bromide method(MTT) was adopted to evaluate the effect of dichloromethane sites of raw and vinegar-processed products on cell activity of NRK and BRL.The levels or contents of aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP),glutathione(GSH),lactate dehydrogenase(LDH),blood urea nitrogen(BUN) were determined in cell culture supernatant and splitting supernatant for evaluation of their oxidative damage effect. Result:Compared with the blank group,dichloromethane site of raw products could obviously inhibit the cell activity of NRK and BRL,and increase the levels of AST,ALT,ALP and LDH(P<0.05,P<0.01) in BRL and decrease the content of GSH;meanwhile,it could obviously increase the levels of LDH and BUN(P<0.05,P<0.01) and decrease the content of GSH in NRK.Compared with corresponding dose group of raw products,vinegar-processed products could improve cell activity of NRK and BRL,and decrease the levels of AST,ALT,ALP and LDH in BRL and increase the content of GSH;also,it decreased the levels of LDH and BUN and increased the content of GSH in NRK. Conclusion:Processing with vinegar can attenuate the hepatotoxicity and nephrotoxicity on NRK and BRL caused by dichloromethane site of Genkwa Flos,it can improve hepatic and renal function and antioxidant capacity.