混合模式色谱分离材料的研究及其应用进展

近年来混合模式色谱以其独特的分离特性受到人们越来越多的关注。混合模式色谱的种类主要集中在反相/离子交换混合模式色谱(reversed-phase/ion-exchange mixed-mode chromatography, RPLC/IEX),亲水作用/离子交换混合模式色谱(hydrophilic interaction/ion-exchange mixed-mode chromatography, HILIC/IEX),反相/亲水作用混合模式色谱(reversed-phase/hydrophilic interaction mixed-mode chromatography, RPLC/HILIC)等混合模式。两种或多种机理混合使用,往往在分离选择性和色谱峰形等方面能得到不同于单一模式操作所得到的效果,分离选择性以及色谱峰形等都能得到极大的改善与提高,这使得混合模式色谱渐渐进入研究者们的视野。混合模式色谱的研究多数集中在色谱填料的设计。混合模式色谱填料的应用主要针对生物样品的分离分析。该文综述了近年来混合模式色谱的研究及其应用进展，并展望了混合模式色谱的发展。

Development and application of separation materials for mixed-mode chromatography

Mixed-mode chromatography has received more and more attention due to its unique chromatographic characteristics recently. In this review, a summary of the development and applications of mixed-mode chromatography is presented. According to the types of hydrophilic interaction/ion-exchange mixed-mode chromatography (HILIC/IEX), reversed-phase/hydrophilic interaction mixed-mode chromatography (RPLC/HILIC) and reversed-phase/ion-exchange mixed-mode chromatography (RPLC/IEX), the preparation and applications of each type are introduced. Compared with single mode chromatography, the selectivity as well as the sample loading capacity of the mixed-mode chromatography can be improved. The researches mostly focus on the design of mixed-mode stationary phases and the applications especially to bio-analysis. The mixed-mode chromatography will be a potent approach for the analysis and separation of complex samples, and it will also be an effective and complementary tool in practical separation work.