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| 豚鼠耳蜗外毛细胞凋亡时听力变化的实验观察 | |
| 引用本文： | 巴云鹏,董明敏,董民声,汤文学,王明臣,邢国兰.豚鼠耳蜗外毛细胞凋亡时听力变化的实验观察[J].中国现代医学杂志,2001,11(10):45-46. |
| 作者姓名： | 巴云鹏 董明敏 董民声 汤文学 王明臣 邢国兰 |
| 作者单位： | 1. 河南医科大学第一附属医院耳鼻咽喉科,郑州,450052 2. 河南医科大学生化教研室,450052 3. 新乡市中心医院,453000 |
| 摘 要： | 目的观察耳蜗外毛细胞发生凋亡时听力改变情况.方法对20只豚鼠药物造模,诱发耳蜗外毛细胞发凋亡.应用TUNEL技术观察凋亡表达,测试ABR阈值观察听力变化.结果应用丁胺卡那霉素1天即可诱发豚鼠耳蜗外毛细胞发生凋亡,连续应用3d,耳蜗外毛细胞凋亡呈强阳性表达,但ABR阈值无明显改变;随着用药时间延长,凋亡细胞数目增加,甚至出现部分毛细胞缺失现黎,此时ABR阈值明显升高.结论耳蜗外毛细胞发生凋亡早期对豚鼠听力无明显影响,随着耳毒性药物应用时间延长,豚鼠ABR阈值升高可能存在两种原因毛细胞凋亡或毛细胞坏死. |
| 关 键 词： | 丁胺卡那霉素 毛细胞 凋亡 TUNEL |
| 修稿时间： | 2000年9月15日 |
Effect of Outer Hair Cell Apoptosis on Hearing Loss in Guinea Pig Cochlea |
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| Ba Yunpeng,Dong Mingmin,Dng Minsheng,Tang Wenxue.Effect of Outer Hair Cell Apoptosis on Hearing Loss in Guinea Pig Cochlea[J].China Journal of Modern Medicine,2001,11(10):45-46. | |
| Authors: | Ba Yunpeng Dong Mingmin Dng Minsheng Tang Wenxue |
| Affiliation: | Ba Yunpeng,Dong Mingmin,Dng Minsheng,Tang Wenxue Department of Otolarynegology,the First Affiliated Hospital of Henan Medical University,Zhengzhou 450052 |
| Abstract: | Objective:To investigate the influence of hearing loss of outer hair cell apoptosis in guinea pig cochlea.Methods:Apoptosis of outer hair cell in 20 guinea pigs was induced by drugs,TdT mediated Peroxidase dUTP nick end labeling (TUNEL) techniques were used to detect the apoptosis and measure the ABR threshold.Results:the morphological changes of apoptosis were noted at 1d with amikacin,there were special morphological changes at 3ds with drugs,but ABR has no bvious change.There was an elevation of ABR at 6 successive ds with drugs.Conclusions:There was no considerable chenges in guinea pigs hearing at the early time of apoptosis.Two reasons may contribute to the elevation of ARB threshold at 6 successive with drugs:1) outer hair cell apoptosis;2) outer harir cell necrosis. |
| Keywords: | Amikacin Hair Cell Apoptosis TUNEL |
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