苍术遗传结构的RAPD分析

 目的考察苍术遗传特性,探讨不同产地苍术遗传多样性。方法用RAPD方法,选择20个随机引物对47株苍术叶片DNA进行扩增,在种内多态性水平、个体间遗传关系及居群间遗传分化3个方面对苍术的遗传结构进行分析。结果①RAPD共检测到94条谱带,其中77个位点为多态位点。苍术种内多态性百分率为81.91%,Shannon′s信息指数0.413 2,Nei′s基因多样性指数为0.274 3;②来源于两个亚居群的11个苍术道地药材聚为一类,而其他居群或亚居群的非道地苍术个体均没有各自聚为一类;③苍术居群内、亚居群间和居群间变异分别占总变异的76.74%,11.58%和11.68%。结论本研究所用3个指标均表明苍术种内遗传多样性水平较高;苍术的遗传变异主要分布在居群内,但居群间已形成一定的遗传分化;茅山苍术个体间的遗传距离较小,遗传背景较为相似,并且不论在居群水平还是个体水平,茅山苍术都能单独聚为一类,表明茅山苍术在长期适应环境的过程中,已经发生遗传上的分化。

RAPD Analysis on Genetic Structure of Atractylodes lancea

OBJECTIVE To study the genetic structure of Atractylodes lancea and discuss its effect on the genetic polymorphism of geoherbs,Mao-cangzhu.METHODS 47 individuals from 3 populations,which were divided into 5 nongeoherb sub-populations and 2 geoherbs sub-populations,of A.lancea were analyzed by RAPD with 20 random primers.The genetic structure of A.lancea was study according to the polymorphism,here and genetic mutation.RESULTS ①77 bands were polymorphic among the total of 94, pps was 81.91%,I was 0.413 2 and he was 0.274 3 in A.lancea.②All of the 11 individuals of goherbs,which included sub-population 6 and 7,clustered together.③Nested analysis showed variations within populations,between sub-populations and between populations of A.lancea were 76.74%,11.58% and 11.68%,respectively.CONCLUSION There are high genetic diversity in A.lancea.Then the major of the genetic variation are distributed in populations,all though there are obviously genetic variation between populations.Finally,genetic distances among the individuals of Mao-cangzhu,which are the geoherbs of A.lancea,were small.Mao-cangzhu can cluster together both in individual and population level.Genetic factors play an important role in forming Mao-cangzhu geoherbs and there are genetic variations between Mao-cangzhu and the others of A.lancea during the long term adaptation to the environment.