双氢青蒿素对卵巢上皮性癌发展和转移的影响

目的：研究双氢青蒿素（DHA）对体外培养的卵巢上皮性癌高度转移细胞株HO-8910pm黏附、侵袭能力以及体内生长的影响，并探讨其相关分子作用机制。方法：用不同浓度DHA处理HO-8910pm，采用基质胶贴壁黏附法检测DHA对癌细胞体外黏附能力的影响；用跨膜小室模型和划痕实验检测DHA对癌细胞侵袭和迁徙能力的影响。用裸鼠的HO-8910pm原位接种卵巢癌模型，检测DHA对卵巢癌侵袭和转移的影响。Western blot法检测DHA对癌细胞聚焦黏附激酶（FAK）磷酸化、MMP-2和MMP-9的体外影响。结果：（1）DHA作用于HO-8910pm细胞后，细胞增殖能力显著抑制，体外黏附和侵袭能力显著下降；（2）体内实验中，DHA可显著抑制卵巢癌腹膜转移；HO-8910pm细胞p-FAK和MMP-2蛋白水平降低，但MMP-9蛋白水平无显著降低。结论：DHA对卵巢上皮性癌细胞的增殖、黏附、侵袭能力及肿瘤转移有-定的抑制作用，其具体机制可能与抑制p-FAK、MMP.2表达水平有关。

Dihydroartiminisin inhibits the growth and metastasis of epithelial ovarian cancer

Objective ：To investigate the capacity of Dihydroartiminisin （DHA） inhibiting the development and metastasis of ovarian cancer. Methods：The adhesion, invasion, and migration of human ovarian cancer cell line （HO-8910pm） was determined following DHA treatment in vitro, using Matrigel coated plate and transwell membrane chamber models, respectively. A mouse ovarian cancer model was established by orthotopic inoculation of HO-8910pm cell line in nude mice. The growth and metastasis in vivo was determined 8 weeks post-implantation in response to DHA treatment. The expression of phosphorylated focal adhesion kinase （p-FAK） and matrix metallo-proteinases （MMP-2 and MMP-9） was evaluated using Western blotting. Results：DHA inhibits ovarian cancer cell proliferation, adhesion, and invasion in vitro in a dose dependent manner, consistent with decreased expression of p-FAK and MMP-2, but not MMP-9. DHA inhibited metastasis significantly in vivo. Conclusion： DHA inhibits the pro- liferation,invasion and adhesion of HO-8910pm ovarian cancer cell line, and suppresses the metastasis in vivo.in hart via down-regulating p-FAK,MMP-2 pathway.