| D-101大孔吸附树脂分离纯化橘皮中的黄酮类物质 |
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| 引用本文： | 胡志军,郝利君,王南溪,陈建秋.D-101大孔吸附树脂分离纯化橘皮中的黄酮类物质[J].食品科学,2010,31(8):65. |
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| 作者姓名： | 胡志军 郝利君 王南溪 陈建秋 |
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| 作者单位： | 中国药科大学分析化学教研室; |
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| 基金项目： | 江苏省环境工程重点实验室开放基金项目(KF2007006) |
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| 摘 要： | 目的:研究D-101大孔吸附树脂分离纯化橘皮黄酮类物质的工艺条件。方法:采用静态和动态吸附-解吸附两种方法,以黄酮类物质吸附率和解吸附率为评价指标,考察橘皮提取液pH值、吸附液料比、静置吸附时间、洗脱液种类和洗脱液料比等影响因素。结果:D-101大孔吸附树脂分离纯化橘皮黄酮类物质的最佳工艺条件为提取液pH4.43,吸附液料比15:1(黄酮类物质溶液:大孔吸附树脂,mL/g)、静置吸附时间90min、洗脱液为95%乙醇溶液、洗脱液料比25:1(95%乙醇溶液:大孔吸附树脂,mL/g)。结论:该方法简单、可行,能够用来分离纯化橘皮中黄酮类物质。
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| 关 键 词： | 橘皮 黄酮类物质 D-101大孔吸附树脂 静态吸附 动态吸附 |
Purification of Crude Total Flavonoids Extracted from Citrus Peel Using D-101 Macroporous Adsorption Resin |
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| HU Zhi-jun,HAO Li-jun,WANG Nan-xi,CHEN Jian-qiu.Purification of Crude Total Flavonoids Extracted from Citrus Peel Using D-101 Macroporous Adsorption Resin[J].Food Science,2010,31(8):65. |
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| Authors: | HU Zhi-jun HAO Li-jun WANG Nan-xi CHEN Jian-qiu |
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| Affiliation: | HU Zhi-jun,HAO Li-jun,WANG Nan-xi,CHEN Jian-qiu(Department of Analytical Chemistry,China Pharmaceutical University,Nanjing 210009,China) |
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| Abstract: | To propose an optimal purification process of total flavonoids in 70% ethanol extract from citrus peel,the static and dynamic adsorption-desorption performance of D-101 macroporous adsorption resin towards total flavonoids from citrus peel was investigated.Major process parameters including pH of sample solution(ethanol extract of citrus peel),sample solution volume/D-101 resin weight ratio(mL/g),standing time and kind and amount(desorption solvent volume/D-101 resin weight ratio,mL/g) of desorption solvent... |
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| Keywords: | citrus peel total flavonoids D-101 macroporous adsorption resin static adsorption dynamic adsorption |
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