血清 ET SOD MDA NO与突发性聋的相关研究

目的:通过对突聋病人血中一氧化氮(NO)、内皮素(ET)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量的检测,探讨突聋与血管收缩因子内皮素、血氧自由基和自由基的清除剂超氧化物歧化酶之间的关系。方法:采用硝酸还原酶法测定了30例突聋患者血中NO含量,硫代巴比妥酸比色法测定MDA含量,放射免疫学方法测定ET含量,黄嘌呤氧化酶法测定SOD含量;并以30例同期体检正常的健康人为对照组。结果:应用金纳多、三磷酸腺苷辅酶A胰岛素、钙阻滞剂(克林奥即马来酸桂哌齐特),联合静脉输入,突聋各组的听力均有不同程度提高,有效率在78.57%以上。治疗后同对照组相比,血清ET、NO、MDA水平明显低于患病之初,而SOD活性明显高于治疗之前P<0.01。结论:检测突聋患者血中ET、SOD、MDA、NO的含量,能帮助研究突发性聋(突聋)的发病机理,估计预后。

Association of The Serum ET, SOD, MDA and NO with Sudden Deafness

Objective:To detect the levels of serum nitric oxide(NO),endothelin(ET),malondialdehyde(MDA) and super oxide dismutase(SOD) in patients with sudden deafness,and investigate the relationship between sudden deafness and ET,SOD,NO,MDA.Methods:30 patients with sudden deafness and 30 normal controls were recruited in this study.The level of NO in serum was detected by nitrate reductase test,that of MDA by thiobarbituric acid test,that of ET by radioimmunity method and that of SOD by xanthine oxidase test.Results:With intravenous ginaton,adenosine disodium triphophate and cinepazide maleate,the hearing in sudden deafness group was improved.The total effective rate was above 78.57%.Compared with controls,the levels of ET,NO and MDA were lower and the activity of SOD was higher in sudden deafness group after treatment than those in sudden deafness group before treatment (P<0.01).Conclusion:Determination of ET,SOD,MDA and NO is helpful to research in the pathogenesis and prognosis of sudden deafness.