抗人肝再生增强因子单克隆抗体的研制

目的　利用纯化的重组人肝再生增强因子(hALR)制备抗hALR的单克隆抗体，建立hALR的检测方法。方法 采用杂交瘤技术制备单克隆抗体，经ELISA和免疫印迹证明其特异性。结果获得了4株分泌抗hALR特异性抗体的杂交瘤 细胞系；无血清培养液效价为1×10-2,腹水效价为1×10-5；亚类鉴定表明2株单克隆抗体为IgGl,另2株为IgG2b；免疫印迹 显示抗体结合抗原的分子量与hALR相符，为特异性抗hALR抗体。结论通过杂交瘤技术，获得了抗hALR的单克隆抗体 为以后的工作奠定了基础。

Preparation and identification of McAbs against human augmenter of liver regeneration

Objective This study was designed to prepare monoclonal antibodies against human augmenter of liver re- generation (hALR),and establish a sensitive assay for it. Methods Monoclonal antibodies were prepared by hybridoma tech- nique. Results Four hybridoma,which stably produced monoclonal antibodies (McAbs) against human augmenter of liver re- generation (hALR),were established by fusion of SP2/0 myeloma cells and splenocytes from Balb/c mice immunized by re- combinant hALR. The titers of specific antibody in culture supernatants and ascites fluid were 1×10-2and 1×10-5 respective- ly by indirect enzyme linked immunosorbent assay (ELISA). The four McAbs belonged to IgG1 and IgG2b subclasses respec- tively and were shown to be reactive with hALR,but not with other cytokines (G-CSF,GM-CSF and IL-6) by ELISA. The results of immunoblotting analysis indicated that the four McAbs all reacted with hALR. Conclusion These antibodies were able to specifically react with hALR,and could be further used in other study.