捻转补泻手法对应激性高血压大鼠CGRP、NO含量的影响

目的:探讨捻转补泻手法对应激性高血压大鼠的效应差异及其作用机制.方法:雄性Wistar大鼠60只,随机分为5组,每组12只,分别为正常组(A)、模型对照组(B)、针刺对照组(C)、针刺补法组(D)、针刺泻法组(E).应激性高血压大鼠造模成功后,C组针刺左侧"太冲"穴,不行针,不做手法;D组针刺左侧"太冲"穴并用捻转补法;E组针刺左侧"太冲"穴并用捻转泻法.A、B两组大鼠不做针刺处理.7日后观察各组大鼠血浆及下丘脑降钙素基因相关肽(CGRP)、一氧化氮(NO)含量的变化.结果:针刺处理后,对大鼠血浆、下丘脑CGRP含量的影响:B组血浆CGRP含量为(261.0±20.1)pg/mL、下丘脑CGRP含量为(156.0±21.8)pg/mg,C组分别为(255.3±33.6)pg/mL、(154.3±47.3)pg/mg,两组与A组的(375.9±36.5)pg/mL、(213.6±50.1)pg/mg比较,CGRP含量下降(均P<0.05);E组的(465.9士53.2)pg/mL、(250.74±47.7)pg/mg与C组及D组的(283.3±29.8)pg/mL、(164.6±47.4)pg/mg比较,CGRP含量明显升高(均P<0.05).C组与B组、D组比较,CGRP含量无明显变化(均P>0.05).对大鼠血浆、下丘脑NO含量的影响:B组分别为(43.7±5.5)/μmol/L、(23.3±2.9)μmol/g,C组为(46.5±6.4)μmol/L、(23.1±3.3)/μmol/g,D组为(45.8±6.7)μmol/L、(25.0±2.7)μmol/g,3组分别与A组的(64.5±9.9)μmol/L、(30.7±1.6)μmol/g比较,NO含量下降(均P<0.05);E组的(55.9±4.6)μmol/L、(30.4±2.1)μmol/g与C组及D组比较,N0含量升高(均P<0.05).C组与B组、D组比较,NO含量变化差异无统计学意义(均P>0.05).结论:针刺泻法可以有效地升高应激性高血压大鼠CGRP、NO含量,从而降低其血压,针刺补法对应激性高血压大鼠降压作用不明显.捻转补法与泻法具有不同的效应.

Effects of reinforcing and reducing methods by twirling and rotating the needle on contents of CGRP and NO in rats with stress-induced hypertension

Objective To explore the different effects of reinforcing and reducing methods by twirling and rotating the needle on stress-induced hypertension and its mechanism.Methods Sixty male Wistar rats were randomly divided into a nomal group(group A),a model group(group B),an acupuncture control group(group C),an acupuncture with reinforcing method group(group D)and an acupuncture with reducing method group(group E),12 rats in each group.The model of stress-induced hypertension was established by foot-shock and n...