不同剂量X射线对人外周血有核细胞DNA及精子DNA损伤的比较

目的:应用单细胞凝胶电泳技术检测不同剂量X射线对人离体外周血有核细胞DNA及精子DNA的损伤,评估外周血有核细胞及精子在高剂量X射线照射后DNA的损伤程度.方法:采用血常规正常的人外周血和采集精液常规正常的人精液,用能量为6MV的X射线给予0Gy,2Gy,4Gy,6Gy,8Gy,10Gy的剂量照射.照射后1h内进行单细...

The DNA Damage of High Doses of X-ray on Human Peripheral Blood Nucleated Cell's and Sperm

Objective To detect the DNA damage of high doses of X-ray on human peripheral blood nucleated cell’s and sperm by single cell gel electrophoresis(SCGE).Evaluation the level of DNA damage of human peripheral blood nucleated cell’s and sperm after high doses of X-ray.Methods Using human peripheral blood with normal blood routine and normal sperm,give the dose of 0Gy,2Gy,4Gy,6Gy,8Gy,10Gy X-ray radiation with energy of 6MU.Detect the percentage of comet-like tail,tail length and content of DNA in tail of whole blood cell’s DNA and sperm’s DNA by SCGE technique in 1 hour.Results The peripheral blood nucleated cell’s and sperm’s comet rate were 1.00±0.10%,2.1±1.5%,respectively,have an evidently variance in 0Gy group(υ=18,t=2.31>1.734,P<0.05).The peripheral blood nucleated cell’s and sperm’s comet rate were 27.10±4.71%,47.37±8.32%,respectively,have a notably variance in 2Gy group(υ=18,t=6.76>1.734,P<0.05).The peripheral blood nucleated cell’s and sperm’s comet rate were 76.32±10.28%,92.22±9.02%,respectively,have an obviously variance in 4Gy group(υ=18,t=3.68<1.734,P<0.05).The peripheral blood nucleated cell’s and sperm’s comet rate were 94.36±3.24%,100%,respectively,have an apparently variance in 6Gy group(υ=18,t=5.508>1.734,P<0.05).The peripheral blood nucleated cell’s and sperm’s comet rate were all 100%,100%,have no-statistical significance in 8Gy,10Gy group.Conclusion The evidence is powerful enough.That the sperm’s SCGE is more sensitive than peripheral blood nucleated cell’s SCGE in reflect the X-ray damage in a certain extent(2~6Gy).