中国成都汉族和大理白族群体D3S1545基因座的遗传多态性研究

为了解我国成都汉放肆本和大理白族群体Ｄ３Ｓ１５４５基因座的遗传多态性，获得中国汉族和大理白族Ｄ３Ｓ１５４５基因座的群体遗传学数据。采集了１４８名无血缘的中国成都汉族个体和９６名无血缘关系的大理白族个体的ＥＤＴＡ抗凝血ｈｅｌｅｘ法提取ＤＮＡ。ＰＣＲ扩增样本ＤＮＡ，聚丙烯酰胺凝胶水平电泳分开。结果显示：在中国成都汉群体中，Ｄ３Ｓ１５４５共发现９个等位基因，等位基因频率为Ｄ３Ｓ１５４６＊８；０．０６５２

Genetic polymorphism at D3S1545 locus in Chengdu Han and Dali Bai populations in China]

The objective of this study was to understand the distribution of the genetic polymorphisms of STR locus D3S1545 in Chinese populations. EDTA-blood specimens were collected from 148 healthy unrelated Han individuals in Chengdu and 96 healthy unrelated Bai individuals in Dali. The DNA samples were extracted using Chelex method, and then they were amplified by PCR technique. PAGE horizontal electrophoresis was used for typing the PCR product. The results there were nine alleles at D3S1545 locus in Chengdu Han population and six alleles at D3S1545 locus in Dali Bai population. The allele frequencies of Chengdu Han population were D3S1545 * 8:0.0642; D3S1545 * 9:0.0034; D3S1545 * 10:0.0068; D3S1545 * 11:0.0203; D3S1545 * 12:0.1824; D3S1545 * 13:0.3075; D3S1545 * 14:0.3243; D3S1545 * 15:0.0844; D3S1545 * 16:0.0068; and 23 genotypes were observed. The allele frequencies of Dali Bai population were D3S1545 * 8:0.00781; D3S1545 * 11:0.0261; D3S1545 * 12:0.1719; D3S1545 * 13:0.3906; D3S1545 * 14:0.2448; D3S1545 * 15:0.0885; and 16 genotypes were observed. The results of test for Hardy-Weinberg equilibrium showed that the genotype distributions observed at the populations were correspondent with the expected. These findings suggest that the allele frequencies at D3S1545 locus in Chengdu Hans have not remarkable different from those in the Bai population of Dali. D3S1545 may be a useful marker for paternity test and personal identification of biological stain.