Inhibition of Ca2+-dependent contraction in swine carotid artery by myosin kinase inhibitors. |
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Authors: | C J Wingard R A Murphy |
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Affiliation: | Department of Molecular Physiology and Biological Physics, University of Virginia Health Science Center, Charlottesville 22906-0011, USA. cwingard@mail.mcg.edu |
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Abstract: | Experiments were designed to examine the efficacy of the MLCK inhibitors wortmannin and ML-9 in intact smooth muscle to determine whether contractile agonists can induce a Ca(2+) and myosin light chain phosphorylation-independent contraction. Both wortmannin and ML-9 reduced active stress in a dose-dependent manner. Both inhibitors interfered with Ca2+ mobilization in either the K(+)-depolarized or agonist activated swine carotid media at concentrations greater than 10 microM. Wortmannin reduced MRLC phosphorylation and stress to resting levels in stimulated tissues while Ca2+ remained above resting levels. There was no evidence for Ca2+ and MRLC phosphorylation-independent stress generation in swine arterial smooth muscle. |
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